Chen Xin, Guo Sheng, Wang Jie-Mei, Li Jie, Ni Liang, Kang Hong-Jie, Qian Da-Wei, Duan Jin-Ao
National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine/Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National Administration of Traditional Chinese Medicine Key Laboratory for Chinese Medicine Resources Recycling Utilization, Nanjing University of Chinese Medicine Nanjing 210023, China.
Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2024 Aug;49(16):4521-4531. doi: 10.19540/j.cnki.cjcmm.20240414.103.
The multivariate statistical analysis was performed to compare the therapeutic effects of Lycii Fructus from different origins on the retinal degenerative diseases(RDD) in mice. The mouse model of RDD was established by intraperitoneal injection of NaIO_3, and the visual function and retinal apoptosis were assessed by dark-light transition and TUNEL assay. Retinal thickness was measured by fundus optical coherence tomography(OCT), and the levels of antioxidant, inflammatory, and angiogenic markers in the serum and eyeball were determined. The therapeutic effects were compared by hierarchical cluster analysis, principal component analysis, and partial least squares-discriminant analysis. The results showed that the extracts of Lycii Fructus from different origins reversed NaIO_3-induced visual damage and retinal apoptosis, reduced oxidative stress, and restored the expression of inflammatory mediators and angiogenic markers in mice. The multivariate statistical analysis based on 17 pharmacodynamic indices suggested that the extract of Lycii Fructus from Ningxia demonstrated better therapeutic effects on RDD than the samples from the other four origins. The results of this study provide a scientific basis for the selection of the advantageous production region of Lycii Fructus for the prevention and treatment of RDD.
进行多变量统计分析以比较不同产地枸杞对小鼠视网膜退行性疾病（RDD）的治疗效果。通过腹腔注射碘酸钠建立RDD小鼠模型，并通过明暗转换和TUNEL检测评估视觉功能和视网膜细胞凋亡。用眼底光学相干断层扫描（OCT）测量视网膜厚度，并测定血清和眼球中抗氧化、炎症和血管生成标志物的水平。通过层次聚类分析、主成分分析和偏最小二乘判别分析比较治疗效果。结果表明，不同产地枸杞提取物可逆转碘酸钠诱导的视觉损伤和视网膜细胞凋亡，减轻氧化应激，并恢复小鼠炎症介质和血管生成标志物的表达。基于17个药效学指标的多变量统计分析表明，宁夏枸杞提取物对RDD的治疗效果优于其他四个产地的样品。本研究结果为选择枸杞防治RDD的优势产区提供了科学依据。
