Department of Molecular Chemistry, Univ. Grenoble-Alpes, CNRS, DCM UMR 5250, CS 40700, 38058, Grenoble Cedex 9, France.
Chemphyschem. 2021 Nov 4;22(21):2231-2239. doi: 10.1002/cphc.202100469. Epub 2021 Aug 23.
Among non-covalent bonds, the host-guest interaction is an attractive way to attach biomolecules to solid surfaces since the binding strength can be tuned by the nature of host and guest partners or through the valency of the interaction. For that purpose, we synthesized cyclodecapeptide scaffolds exhibiting in a spatially controlled manner two independent domains enabling the multimeric presentation of guest molecules on one face and the other face enabling the potential grafting of a biomolecule of interest. In this work, we were interested in the β-cyclodextrin/ferrocene inclusion complex formed on β-CD monolayers functionalized surfaces. By using surface sensitive techniques such as quartz crystal microbalance and surface plasmon resonance, we quantified the influence of the guest valency on the stability of the inclusion complexes. The results show a drastic enhancement of the affinity with the gradual increase of guest valency. Considering that the sequential binding events are equal and independent, we applied the multivalent model developed by the Huskens group to extract intrinsic binding constants and an effective concentration of host.
在非共价键中，主体-客体相互作用是将生物分子附着到固体表面的一种有吸引力的方法，因为结合强度可以通过主体和客体伙伴的性质或通过相互作用的价来调节。为此，我们合成了环十肽支架，以空间控制的方式展示了两个独立的结构域，使客体分子在一个面上进行多聚体呈现，而另一面则能够潜在地接枝感兴趣的生物分子。在这项工作中，我们对β-环糊精/二茂铁包合物在β-CD 单层功能化表面上的形成感兴趣。通过使用石英晶体微天平和表面等离子体共振等表面敏感技术，我们量化了客体价态对包合物稳定性的影响。结果表明，随着客体价态的逐渐增加，亲和力得到了显著增强。考虑到顺序结合事件是相等且独立的，我们应用了 Huskens 小组开发的多价模型来提取固有结合常数和主体的有效浓度。
