Onori G
Dipartimento di Fisica dell'Università, Perugia, Italy.
Biophys Chem. 1987 Dec;28(3):183-90. doi: 10.1016/0301-4622(87)80088-1.
The interaction of copper(II) with adenosine triphosphate (ATP) has been studied as a function of pH in the range pH 3-12. Our approach is the study of the effect of binding both on the ATP ultraviolet absorption spectrum and on the optical d-d transition of copper ions. The results show that Cu(II)-ATP complexes exist in a variety of forms in equilibrium, the percentage of each species varying according to the state of ionization of the intrinsic adenine, phosphate and ribose groups. These results also show a close correlation between the rate of dephosphorylation of ATP in the presence of Cu(II) ions and Cu(II) bonding to the adenine of ATP, thus supporting the hypothesis that the metal-ion/nucleic-base interactions are crucial for the observation of a metal-ion promoted dephosphorylation of ATP (D.H. Buisson and H. Sigel, Biochim. Biophys. Acta 343 (1974) 45).
研究了在pH值为3至12范围内，铜（II）与三磷酸腺苷（ATP）的相互作用与pH的关系。我们的方法是研究结合对ATP紫外吸收光谱以及铜离子光学d-d跃迁的影响。结果表明，Cu（II）-ATP络合物以多种形式处于平衡状态，每种物种的百分比根据内在腺嘌呤、磷酸和核糖基团的电离状态而变化。这些结果还表明，在存在Cu（II）离子的情况下，ATP的去磷酸化速率与Cu（II）与ATP腺嘌呤的结合之间存在密切相关性，从而支持了金属离子/核酸碱基相互作用对于观察金属离子促进的ATP去磷酸化至关重要的假设（D.H. Buisson和H. Sigel，《生物化学与生物物理学报》343（1974）45）。
