Toulme J J
Bioinorg Chem. 1978 Apr;8(4):319-29. doi: 10.1016/s0006-3061(00)80165-9.
Spectrophotometric studies have provided evidence for zinc-mediated ternary complexes between ATP and aromatic amino acids. The hypochromicity observed in the 260 nm band of ATP increased in the order phenylalanine less than tyrosine less than tryptophan. Adding alanine did not produce any change of the ATP spectrum. The association constant was four fold higher for the ATP-Zinc-Tryptophan complex than for that of the ATP-Zinc-Alanine. The increased stability of the former complex was ascribed to the stacking interaction between indole and adenine rings. The maximum concentration of the ATP-Zinc-Tryptophan complex occurred at about pH 8.0. For these ternary complexes several possible stacked structures involving or not involving N(7) of adenine are discussed.
分光光度研究为锌介导的ATP与芳香族氨基酸之间的三元复合物提供了证据。在ATP的260nm波段观察到的减色效应按苯丙氨酸＜酪氨酸＜色氨酸的顺序增强。添加丙氨酸不会使ATP光谱产生任何变化。ATP-锌-色氨酸复合物的缔合常数比ATP-锌-丙氨酸复合物的缔合常数高四倍。前一种复合物稳定性的增加归因于吲哚环与腺嘌呤环之间的堆积相互作用。ATP-锌-色氨酸复合物的最大浓度出现在pH约8.0处。对于这些三元复合物，讨论了几种可能的堆积结构，这些结构涉及或不涉及腺嘌呤的N(7)。
