Greenaway F T, Ledbetter J W
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425.
Biophys Chem. 1987 Dec;28(3):265-71. doi: 10.1016/0301-4622(87)80097-2.
The fluorescence lifetime and depolarization of the pyridoxal 5'-phosphate label demonstrated different environments at the structure-solvent interface for micelles, liposomes, proteins and membranes. A short lifetime and rotational correlation time for the micelles and liposomes proved that the label was strongly associated with the water solvent and rotated freely about the covalent bond. The proteins provided a more buried or hydrophobic site as shown by an increase in the lifetimes. Rotational correlation times of 4-6 ns for sarcolemma and erythrocyte membranes suggested restricted rotation for the pyridoxal 5'-phosphate label. Lower values of the rotational correlation time for rod outer segment and myelin sheath proved that the protein epsilon-amino groups are at the solvent interface which allows for more rotation.
磷酸吡哆醛标记物的荧光寿命和去极化表明，在胶束、脂质体、蛋白质和膜的结构-溶剂界面处存在不同的环境。胶束和脂质体的荧光寿命短且旋转相关时间短，这证明该标记物与水溶剂强烈结合，并围绕共价键自由旋转。蛋白质提供了一个更隐蔽或疏水的位点，这表现为荧光寿命增加。肌膜和红细胞膜的旋转相关时间为4-6纳秒，这表明磷酸吡哆醛标记物的旋转受到限制。杆状外段和髓鞘的旋转相关时间较低，这证明蛋白质ε-氨基位于溶剂界面，从而允许更多的旋转。
