蜂王浆对多囊卵巢综合征大鼠模型激素及氧化变化的改善作用：一项实验研究。
Improved hormonal and oxidative changes by Royal Jelly in the rat model of PCOS: An experimental study.
作者信息
Khazaei Fatemeh, Ghanbari Elham, Khazaei Mozafar
机构信息
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
出版信息
Int J Reprod Biomed. 2021 Jul 27;19(6):515-524. doi: 10.18502/ijrm.v19i6.9373. eCollection 2021 Jun.
BACKGROUND
Polycystic ovarian syndrome (PCOS) is an endocrine and complex metabolic disorder, associated with anovulation, changes in sex hormone, biochemical factors, and ovarian tissue. Royal Jelly (RJ) has antioxidant and anti-inflammatory properties.
OBJECTIVE
To examine the therapeutic effect of RJ on PCOS-related hormonal and biochemical changes in a rat model of PCOS.
MATERIALS AND METHODS
In this experimental study, 42 female Wistar rats (weighing 180-200 gr, aged 10-12 wk) were divided into six groups (n = 7/each): control; PCOS; RJ 100 mg/kg; RJ 200 mg/kg; PCOS + RJ 100 mg/kg; and PCOS + RJ 200 mg/kg. After 21 days, the animals were weighed and dissected. The serums were used for nitric oxide (NO) and ferric-reducing antioxidant power (FRAP) assay and estradiol and progesterone measurements. The ovaries were assessed for histological changes.
RESULTS
PCOS increased estradiol and NO levels, and decreased progesterone and FRAP levels. In PCOS + RJ groups, the progesterone (p = 0.01) and FRAP levels (p 0.001) increased and the estradiol and NO (p 0.001) levels decreased significantly. Moreover, the number of mature follicles (p = 0.01) and corpus luteum increased (p 0.001), and ovarian and uterus weight deceased significantly (p 0.001).
CONCLUSION
RJ improved estradiol, progesterone, FRAP, and NO levels, and ovarian structure in the rat model of PCOS.
背景
多囊卵巢综合征（PCOS）是一种内分泌及复杂的代谢紊乱疾病，与无排卵、性激素变化、生化因素及卵巢组织有关。蜂王浆（RJ）具有抗氧化和抗炎特性。
目的
研究RJ对PCOS大鼠模型中与PCOS相关的激素和生化变化的治疗作用。
材料与方法
在本实验研究中，将42只雌性Wistar大鼠（体重180 - 200克，年龄10 - 12周）分为六组（每组n = 7）：对照组；PCOS组；RJ 100毫克/千克组；RJ 200毫克/千克组；PCOS + RJ 100毫克/千克组；PCOS + RJ 200毫克/千克组。21天后，对动物进行称重并解剖。采集血清用于一氧化氮（NO）和铁还原抗氧化能力（FRAP）测定以及雌二醇和孕酮测量。对卵巢进行组织学变化评估。
结果
PCOS使雌二醇和NO水平升高，孕酮和FRAP水平降低。在PCOS + RJ组中，孕酮（p = 0.01）和FRAP水平（p < 0.001）升高，雌二醇和NO水平（p < 0.001）显著降低。此外，成熟卵泡数量（p = 0.01）和黄体数量增加（p < 0.001），卵巢和子宫重量显著减轻（p < 0.001）。
结论
RJ改善了PCOS大鼠模型中的雌二醇、孕酮水平、FRAP和NO水平以及卵巢结构。
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