一种用于生成和实时监测DNA损伤的激光介导光操纵工具箱。
A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.
作者信息
Madhavan Bindhu K, Han Zhe, Sickmann Albert, Pepperkok Rainer, Nawroth Peter P, Kumar Varun
机构信息
Department of Internal Medicine-I and Clinical Chemistry, University Hospital of Heidelberg, Im Neuenheimer Feld 410, Heidelberg 69120, Germany.
Leibniz Institute for Analytical Sciences, Dortmund 44227, Germany.
出版信息
STAR Protoc. 2021 Aug 2;2(3):100700. doi: 10.1016/j.xpro.2021.100700. eCollection 2021 Sep 17.
With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).
随着基于激光的显微镜工具的发展，现在有可能探索细胞内发生的机械动力学过程。在这里，我们描述了一种先进的实验方案，该方案使用激光诱导DNA损伤来实时研究DNA修复动力学。该方案可用于诱导、测试和研究与DNA双链断裂、链间交联修复和单链断裂修复相关的修复机制。有关该方案使用和执行的完整详细信息，请参考Kumar等人（2017年、2020年）的研究。
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