用于研究发热对小鼠T细胞黏附和迁移影响的发热范围全身热疗（WBH）实验方案。
Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration.
作者信息
Lin ChangDong, Liu ZhaoYuan, Li Yue, Chen JianFeng
机构信息
State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
出版信息
STAR Protoc. 2021 Aug 5;2(3):100720. doi: 10.1016/j.xpro.2021.100720. eCollection 2021 Sep 17.
Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to Lin et al. (2019).
发热是一种复杂的生理反应，可在感染和炎症期间增强免疫监视。发热范围的全身热疗（WBH）治疗可在实验中模拟小鼠的发热状态。在此，我们描述了一种用WBH和正常体温治疗小鼠的方案。我们描述了从小鼠脾脏中分离T细胞，随后评估T细胞的黏附和迁移。这种动物模型可用于研究发热诱导的免疫系统功能障碍。有关本方案使用和实施的完整详细信息，请参考Lin等人（2019年）的研究。
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