College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Mikrochim Acta. 2021 Aug 16;188(9):299. doi: 10.1007/s00604-021-04958-z.
Fluorescence sensing of glutathione by tailor-made chemical sensors is a prospective technique, which could provide simple, fast, and visual detection. Herein, a fluorescence sensor based on vanadium oxide quantum dots (VO QDs) and permanganate (MnO) has been designed for monitoring glutathione. The bifunctional VO QDs, possessing rich redox chemistry and robust fluorescence (exhibiting fluorescence near 505 nm upon excitation at 450 nm), were synthesized via cryogenic-mediated liquid-phase exfoliation. In the presence of MnO, VO QDs induced the spontaneous formation of MnO nanosheets which caused the fluorescence quenching. However, the subsequent introduction of glutathione could trigger MnO reduction to Mn, and the fluorescence was recovered. Based on this phenomenon, an "on-off-on" fluorescence sensor for glutathione detection was established. Under the optimal conditions, this sensor allowed detection of glutathione in the linear range of 0.5-100 μM with a detection limit of 0.254 μM. Additionally, the proposed strategy revealed the selectivity toward glutathione and the potential of practical application in the analysis of human serum, vegetable, and fruit samples.
基于定制化学传感器的谷胱甘肽荧光传感是一种很有前景的技术，可以提供简单、快速和直观的检测。在此，设计了一种基于氧化钒量子点（VO QDs）和高锰酸盐（MnO）的荧光传感器来监测谷胱甘肽。具有丰富氧化还原化学性质和强荧光性（在 450nm 激发时发出近 505nm 的荧光）的双功能 VO QDs 是通过低温介导的液相剥离合成的。在 MnO 的存在下，VO QDs 诱导 MnO 纳米片的自发形成，导致荧光猝灭。然而，随后引入的谷胱甘肽可以触发 MnO 还原为 Mn，荧光恢复。基于这一现象，建立了一种用于谷胱甘肽检测的“开-关-开”荧光传感器。在最佳条件下，该传感器可以在 0.5-100μM 的线性范围内检测谷胱甘肽，检测限为 0.254μM。此外，该策略还表现出对谷胱甘肽的选择性，以及在人血清、蔬菜和水果样本分析中的实际应用潜力。
