State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry , Chinese Academy of Sciences , Changchun , Jilin 130022 , China.
University of Science and Technology of China , Hefei , Anhui 230026 , China.
Anal Chem. 2019 May 7;91(9):5762-5767. doi: 10.1021/acs.analchem.8b05961. Epub 2019 Apr 17.
Glutathione (GSH) can help the body maintain the function of the normal immune system and its level change is associated with a variety of diseases. To achieve the ultrasensitive assay of GSH, a "switch on" nanosensor is designed on the basis of GSH regulating the inner filter effect (IFE) of MnO nanosheets (MnO NS) on boron nitride quantum dots (BNQDs). Here, the fluorescence of BNQDs is quenched efficiently in the presence of redoxable MnO NS because of the superior light absorption capability; however, the introduction of GSH can trigger the decomposition of MnO to Mn and weaken the IFE, causing the partial fluorescence recovery. The recovered fluorescence is dependent on the concentration of GSH. Under the optimal conditions, this sensing platform shows the response to GSH in the range of 0.5-250 μM with the detection limit of 160 nM. On the basis of the GSH activated reduction of MnO NS, the MnO NS/BNQDs nanoprobes exhibit good selectivity to GSH. The practical application of the proposed system is demonstrated by detecting the GSH in human plasma samples with satisfying results.
谷胱甘肽（GSH）可以帮助身体维持正常免疫系统的功能，其水平的变化与多种疾病有关。为了实现对 GSH 的超灵敏检测，基于 GSH 调节氧化锰纳米片（MnO NS）对氮化硼量子点（BNQDs）的内滤效应（IFE），设计了一种“开启”纳米传感器。在这里，由于具有优异的光吸收能力，在存在可还原的 MnO NS 的情况下，BNQDs 的荧光被有效地猝灭；然而，GSH 的引入可以触发 MnO 的分解为 Mn 并减弱 IFE，导致部分荧光恢复。恢复的荧光与 GSH 的浓度有关。在最佳条件下，该传感平台在 0.5-250 μM 的范围内对 GSH 表现出响应，检测限为 160 nM。基于 MnO NS 的 GSH 激活还原，MnO NS/BNQDs 纳米探针对 GSH 表现出良好的选择性。该系统的实际应用通过检测人血浆样品中的 GSH 得到了验证，结果令人满意。
