Wang An-Qi, Li Xi-Wen, Yuan Qing-Jun, Guo Ning, Yang Bin, Sun Yi
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(14):3514-3521. doi: 10.19540/j.cnki.cjcmm.20210430.102.
According to the records of Chinese materia medica,Coptis chinensis var. brevisepala is an authentic Chinese medicinal plant highly recommended by ancient physicians since its rhizome is like a string of beads and has a good medicinal value. However,its medicinal components and values remain to be studied as it is endangered because of overexploitation. Therefore,this study aims to quantitatively determine its effective components based on UPLC-QTOF-MS,and to compare the contents of isoquinoline alkaloids in C.chinensis var. brevisepala with those in other Coptis species. Meanwhile,molecular methods accurately identified 12 batches of C. chinensis var. brevisepala,9 batches of C. chinensis,4 batches of C. deltoidea,and 1 batch of C. teeta. Gradient elution was performed with Waters CORTECS C18 column( 4. 6 mm× 150 mm,2. 7 μm) and the mobile phase acetonitrile-water with 0. 4% formic acid. Mass spectrometry was conducted in ESI positive mode. The quantitative results showed that 8 main alkaloids had a good linear relationship within the concentration range( R~2>0. 996),with the recovery rate of 95. 18%-105. 0% and the RSD of 0. 28%-3. 7%. Compared with that of other Coptis species,the rhizome of C. chinensis var. brevisepala had the highest contents of berberine and columbamine. The total content of the 8 alkaloids in C. chinensis var. brevisepala was similar to that in C. chinensis but higher than that of the other two species. PCA was performed to compare the alkaloids among the 4 species. Besides,the 8 alkaloids were evaluated in different parts of C. chinensis var. brevisepala. The results indicate that this method is reliable and efficient and can provide a reference for the quality research.
据《本草纲目》记载，短萼黄连是一种地道的中药材，因其根茎呈连珠状而备受古代医家推崇，具有较高的药用价值。然而，由于过度采挖，该物种已濒临灭绝，其药用成分和价值仍有待研究。因此，本研究旨在基于超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-QTOF-MS）对其有效成分进行定量测定，并比较短萼黄连与其他黄连属植物中异喹啉生物碱的含量。同时，采用分子方法准确鉴定了12批次的短萼黄连、9批次的黄连、4批次三角叶黄连和1批次云南黄连。采用沃特世CORTECS C18柱（4.6 mm×150 mm，2.7 μm）进行梯度洗脱，流动相为乙腈-水（含0.4%甲酸）。质谱采用电喷雾离子源正离子模式。定量结果表明，8种主要生物碱在浓度范围内具有良好的线性关系（R²＞0.996），回收率为95.18% - 105.0%，相对标准偏差为0.28% - 3.7%。与其他黄连属植物相比，短萼黄连根茎中黄连素和小檗胺的含量最高。短萼黄连中8种生物碱的总含量与黄连相似，但高于其他两个物种。采用主成分分析（PCA）比较了4种黄连属植物中的生物碱。此外，还对短萼黄连不同部位的8种生物碱进行了评价。结果表明，该方法可靠、高效，可为质量研究提供参考。
