[麦冬皂苷D对心肌细胞脂肪酸代谢酶的影响]
[Effects of ophiopogonin D on fatty acid metabolic enzymes in cardiomyocytes].
作者信息
Tang Xiang-Lin, Lin Yi, Wang Yu-Guang, Gao Yue
机构信息
Institute of Radiation Medicine,Academy of Military Medical Sciences Beijing 100850,China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(14):3672-3677. doi: 10.19540/j.cnki.cjcmm.20210311.401.
To explore the effect of ophiopogonin D on main fatty acid metabolic enzymes in human cardiomyocyte AC-16,so as to provide reference for cardiovascular protection mechanism and safe clinical application of Ophiopogon japonicus.CCK-8 (cell counting kit-8) was used to detect the effect of different concentrations of ophiopogonin D on the viability of cardiomyocytes.Meanwhile,the effect of different concentrations of ophiopogonin D on the morphology and quantity of cardiomyocytes was observed under microscope.The effect of ophiopogonin D on the mRNA expression of CYP2J2,CYP4F3,CYP4A11,CYP4A22 and CYP4F2 in cardiomyocytes was detected by RT-PCR.Western blot was used to detect the protein expression of CYP4F3 in different concentrations of ophiopogonin D.Compared with the control group,low-concentration ophiopogonin D had no effect on the viability of cardiomyocytes.However,ophiopogonin D with a concentration of higher than 20μmol·L(-1)could promote the viability.Under the microscope,ophiopogonin D with a concentration of below 100μmol·L(-1)had no significant effect on the morphology and number of cardiomyocytes.RT-PCR results showed that compared with the control group,5μmol·L(-1)ophiopogonin D could slightly up-regulate mRNA expressions of CYP2J2 and CYP4F3,while high-concentration ophiopogonin D (10 and 20μmol·L(-1)) could significantly induce mRNA expressions of CYP2J2and CYP4F3 in a dose-dependent manner (P<0.05).The same concentration of ophiopogonin D had a little effect on the mRNA expressions of CYP4A11,CYP4A22 and CYP4F2.Western blot results showed that 20μmol·L(-1)ophiopogonin D could significantly induce the protein expression of CYP4F3 in a dose-dependent manner (P<0.05).Based on the above results,ophiopogonin D (less than100μmol·L(-1)) has no effect on the viability of AC-16 cardiomyocytes.Ophiopogonin D (less than 100μmol·L~(-1)) can selectively induce the expressions of CYP2J2 and CYP4F3,regulate the metabolic pathway of fatty acid signaling molecules,and thus protecting the cardiovascular system.
探讨麦冬皂苷D对人心肌细胞AC-16中主要脂肪酸代谢酶的影响，为麦冬的心血管保护机制及临床安全应用提供参考。采用CCK-8（细胞计数试剂盒-8）检测不同浓度麦冬皂苷D对心肌细胞活力的影响。同时，在显微镜下观察不同浓度麦冬皂苷D对心肌细胞形态和数量的影响。采用RT-PCR检测麦冬皂苷D对心肌细胞中CYP2J2、CYP4F3、CYP4A11、CYP4A22和CYP4F2 mRNA表达的影响。采用Western blot检测不同浓度麦冬皂苷D对CYP4F3蛋白表达的影响。与对照组相比，低浓度麦冬皂苷D对心肌细胞活力无影响。然而，浓度高于20μmol·L⁻¹的麦冬皂苷D可促进细胞活力。在显微镜下，浓度低于100μmol·L⁻¹的麦冬皂苷D对心肌细胞的形态和数量无明显影响。RT-PCR结果显示，与对照组相比，5μmol·L⁻¹的麦冬皂苷D可轻微上调CYP2J2和CYP4F3的mRNA表达，而高浓度麦冬皂苷D（10和20μmol·L⁻¹）可显著诱导CYP2J2和CYP4F3的mRNA表达，呈剂量依赖性（P＜0.05）。相同浓度的麦冬皂苷D对CYP4A11、CYP4A22和CYP4F2的mRNA表达影响较小。Western blot结果显示，20μmol·L⁻¹的麦冬皂苷D可显著诱导CYP4F3的蛋白表达，呈剂量依赖性（P＜0.05）。基于上述结果，麦冬皂苷D（低于100μmol·L⁻¹）对AC-16心肌细胞活力无影响。麦冬皂苷D（低于100μmol·L⁻¹）可选择性诱导CYP2J2和CYP4F3的表达，调节脂肪酸信号分子的代谢途径，从而保护心血管系统。
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