Department of Biological Sciences and Biotechnology, College of Natural Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Arch Virol. 2021 Nov;166(11):3013-3021. doi: 10.1007/s00705-021-05202-y. Epub 2021 Aug 17.
In recent years, several novel circular single-stranded DNA viruses have been detected in various mammals, birds, insects, and environmental samples using metagenomic and high-throughput sequencing approaches. In this study, we tested for the presence of circoviruses in 243 bat fecal samples collected between 2018 and 2019 from 48 sampling sites across Korea. To detect circoviruses, nested PCR was performed with degenerate primers targeting a conserved replication-associated protein (rep) gene of circovirus/cyclovirus. Among 243 samples tested, a total of 37 fecal samples from 14 sampling sites were PCR-positive for circoviruses at a frequency rate of 15.23%. We obtained 36 partial rep gene sequences of circoviruses and one complete genome sequence of bat-associated circovirus 12, encompassing a genome size of 2097 nt containing two inversely arranged open reading frames and a conserved nonamer sequence in the apex of a stem-loop structure. In addition, we found four bat species that were harboring circoviruses in Korea based on species identification PCR of circovirus-positive bat fecal samples. Detailed sequence analysis indicated that the bat-associated circovirus sequences identified in this study were related to those of known bat and avian groups of circoviruses. Herein, we report evidence for the presence of bat-associated circoviruses in Korean bats.
近年来，采用宏基因组和高通量测序方法，在各种哺乳动物、鸟类、昆虫和环境样本中检测到了几种新型环状单链 DNA 病毒。在这项研究中，我们检测了 2018 年至 2019 年期间从韩国 48 个采样点采集的 243 份蝙蝠粪便样本中是否存在圆环病毒。为了检测圆环病毒，使用针对圆环病毒/环病毒保守复制相关蛋白（rep）基因的简并引物进行巢式 PCR。在测试的 243 个样本中，共有来自 14 个采样点的 37 个粪便样本对圆环病毒呈 PCR 阳性，阳性率为 15.23%。我们获得了 36 个圆环病毒部分 rep 基因序列和一个蝙蝠相关圆环病毒 12 的完整基因组序列，其基因组大小为 2097nt，包含两个反向排列的开放阅读框和一个在茎环结构顶端保守的非九聚体序列。此外，我们还根据圆环病毒阳性蝙蝠粪便样本的圆环病毒种特异性 PCR ，在韩国发现了四种携带圆环病毒的蝙蝠。详细的序列分析表明，本研究中鉴定的蝙蝠相关圆环病毒序列与已知的蝙蝠和禽圆环病毒群有关。本研究报告了韩国蝙蝠中存在蝙蝠相关圆环病毒的证据。
