Buntum Tanawat, Kongprayoon Atchariya, Mungyoi Wanwisa, Charoenram Pimchanok, Kiti Kitipong, Thanomsilp Chuleeporn, Supaphol Pitt, Suwantong Orawan
School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
The Petroleum and Petrochemical College, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
Int J Biol Macromol. 2021 Oct 31;189:135-141. doi: 10.1016/j.ijbiomac.2021.08.083. Epub 2021 Aug 14.
The potential of chitosan nanoparticles (CSNPs) loaded with essential oil (EO) incorporated into semi-solid PVA hydrogels for use in wound management was studied. Two types of essential oil were compared including clove essential oil (CEO) and turmeric essential oil (TEO). The EO-loaded CSNPs were prepared by a two-step method; oil-in-water (o/w) emulsification followed by ionic gelation using different ratios of chitosan:EO (i.e., 1:0.25, 1:0.50, 1:0.75, and 1:1.00 w/w). The increasing amount of EO caused more aggregate structure as observed from SEM images. The TEO-loaded CSNPs showed a higher extent of aggregation than the CEO-loaded CSNPs. The adhesiveness of the semi-solid PVA hydrogels containing TEO-loaded CSNPs was the highest. The use of EO-loaded CSNPs in the semi-solid PVA hydrogel helped to sustain and prolong the release rate of EO from the hydrogels as compared to just the EO alone. The studied semi-solid PVA hydrogels were non-toxic to both NCTC clone 929 and NHDF cells. Overall results suggested that these semi-solid hydrogels are good candidates for use in wound management.
研究了负载精油（EO）的壳聚糖纳米颗粒（CSNPs）掺入半固体聚乙烯醇（PVA）水凝胶中用于伤口处理的潜力。比较了两种精油，即丁香精油（CEO）和姜黄精油（TEO）。负载EO的CSNPs采用两步法制备；先进行水包油（o/w）乳化，然后使用不同比例的壳聚糖：EO（即1:0.25、1:0.50、1:0.75和1:1.00 w/w）通过离子凝胶化法制备。从扫描电子显微镜（SEM）图像观察到，EO含量增加导致聚集结构增多。负载TEO的CSNPs比负载CEO的CSNPs表现出更高程度的聚集。含有负载TEO的CSNPs的半固体PVA水凝胶的粘附性最高。与单独使用EO相比，在半固体PVA水凝胶中使用负载EO的CSNPs有助于维持和延长EO从水凝胶中的释放速率。所研究的半固体PVA水凝胶对NCTC克隆929细胞和人正常皮肤成纤维细胞（NHDF）均无毒。总体结果表明，这些半固体水凝胶是用于伤口处理的良好候选材料。
