Zorzi Bueno Cecilia, Wiggers Helton José, Chevallier Pascale, Copes Francesco, Mantovani Diego
Laboratory for Biomaterials and Bioengineering (LBB-BPK), Associação de Ensino, Pesquisa e Extensão BI-OPARK, Max Planck Avenue, 3797, Building Charles Darwin, Toledo 85919-899, PR, Brazil.
Laboratory for Biomaterials and Bioengineering (LBB-UL), Department of Mining, Metallurgical and Materials Engineering, CHU de Québec Research Center, Division of Regenerative Medicine, Laval University, Quebec City, QC G1V0A6, Canada.
Polymers (Basel). 2025 Aug 21;17(16):2261. doi: 10.3390/polym17162261.
If untreated, skin wounds can lead to severe complications. Depending on the type of injury, long-term antibiotic administration is often required, and this decreases patient compliance. This limitation could be addressed by applying dressings capable of preventing infections by controlling drug release to the wound site. In this research, biodegradable wound dressings were investigated, based on natural polymers chitosan and alginate and incorporating the broad-spectrum gentamicin as antibiotic. Specifically, gentamicin was loaded into alginate nanoparticles, which were then loaded into chitosan-based films. This approach aimed at obtaining a system capable of modulating antibiotic release. The obtained nanoparticles had an average diameter of 86 nm and polydispersity index of 0.15. Antibiotic loading was around 600 µg/mg, with loading efficiency close to 100%. Films incorporating nanoparticles were compared to control films, which contained only gentamicin. Results showed that nanoparticles incorporation decreased film's swelling in phosphate buffer saline, thus leading to a decrease in burst release while cytocompatibility for human dermal fibroblasts was maintained. Antibacterial activity was confirmed against both gram-positive and gram-negative bacteria. Moreover, the antibiotic was released as a function of pH, with distinct behavior at pHs ranging from 7.4 to 5.5. This indicates that alginate nanoparticles dispersed in chitosan films effectively release gentamicin on demand.
如果不进行治疗，皮肤伤口可能会导致严重的并发症。根据损伤类型，通常需要长期使用抗生素，而这会降低患者的依从性。通过应用能够通过控制药物向伤口部位释放来预防感染的敷料，可以解决这一局限性。在本研究中，基于天然聚合物壳聚糖和海藻酸盐，并掺入广谱庆大霉素作为抗生素，对可生物降解的伤口敷料进行了研究。具体而言，将庆大霉素负载到海藻酸盐纳米颗粒中，然后将其负载到基于壳聚糖的薄膜中。这种方法旨在获得一种能够调节抗生素释放的系统。所获得的纳米颗粒的平均直径为86nm，多分散指数为0.15。抗生素负载量约为600μg/mg，负载效率接近100%。将掺入纳米颗粒的薄膜与仅含有庆大霉素的对照薄膜进行比较。结果表明，掺入纳米颗粒会降低薄膜在磷酸盐缓冲盐水中的溶胀，从而导致突释减少，同时保持对人真皮成纤维细胞的细胞相容性。对革兰氏阳性菌和革兰氏阴性菌均证实了抗菌活性。此外，抗生素的释放是pH的函数，在pH值从7.4到5.5范围内表现出不同的行为。这表明分散在壳聚糖薄膜中的海藻酸盐纳米颗粒能够按需有效释放庆大霉素。
