Department of Dental Materials, Army Medical College, National University of Medical Sciences, Islamabad, Pakistan.
Department of Dental Materials, Ayub Medical College, Abbottabad, Pakistan.
PLoS One. 2022 Aug 19;17(8):e0273079. doi: 10.1371/journal.pone.0273079. eCollection 2022.
This study aims to investigate new tissue conditioner (TC) formulations involving chitosan nanoparticles (CSNPs) and essential oils (EO) for their antifungal potential, release kinetics, and hardness.
CSNPs were synthesized, and the separate solutions of CSNP were prepared with two types of EO, i.e., Oregano oil and Lemongrass. The EO was loaded separately in two concentrations (200 μL and 250 μL). The blank and EO-loaded CSNPs were screened against Candida albicans (C. albicans), and their minimum inhibitory concentration was established. GC Reline™ (GC corporation, USA) TC was considered a control group, whereby the four experimental groups were prepared by mixing CSNPs/EO solutions with TC powder. The antifungal effectiveness (C. albicans) and release kinetics behavior (1-6 h, 24 h, 48 h, and 72 h) was investigated. The Shore A hardness of control and experimental groups was evaluated in dry and wet modes (deionized water and artificial saliva). For statistical analysis, SPSS version 22 was used to do a one-way ANOVA post-hoc Tukey's test.
Compared to the control group, TCs containing blank CSNPs and CSNPs loaded with EO showed 3 and 5 log reductions in C. albicans growth, respectively. A significantly high antifungal effect was observed with TC containing lemongrass essential oil (200 μL). The continuous release of EO was detected for the first 6 hours, whereas completely stopped after 48 hours. Mean hardness values were highest for dry samples and lowest for samples stored in artificial saliva. The statistically significant difference within and between the study groups was observed in mean and cumulative essential oils release and hardness values of TCs over observed time intervals irrespective of storage media.
TCs containing essential-oil-loaded CSNPs seem a promising alternative treatment of denture-induced stomatitis, however, a further biological analysis should be taken.
本研究旨在探讨新型组织调节剂（TC）配方，包括壳聚糖纳米粒子（CSNPs）和精油（EO），以研究其抗真菌潜力、释放动力学和硬度。
合成 CSNPs，并制备 CSNP 的两种单独溶液，即牛至油和柠檬草油。EO 分别以两种浓度（200 μL 和 250 μL）加载。筛选空白和负载 EO 的 CSNPs 对白色念珠菌（C. albicans）的最小抑菌浓度。将 GC Reline™（GC 公司，美国）TC 作为对照组，通过将 CSNPs/EO 溶液与 TC 粉末混合制备四个实验组。研究抗真菌效果（白色念珠菌）和释放动力学行为（1-6 小时、24 小时、48 小时和 72 小时）。在干燥和湿润模式（去离子水和人工唾液）下评估对照组和实验组的肖氏 A 硬度。使用 SPSS 版本 22 进行单向方差分析后进行 Tukey 检验。
与对照组相比，含有空白 CSNPs 和负载 EO 的 CSNPs 的 TC 分别使 C. albicans 生长减少 3 和 5 个对数。含有柠檬草精油的 TC 表现出显著高的抗真菌效果。EO 的持续释放在最初 6 小时内被检测到，而在 48 小时后完全停止。干燥样品的平均硬度值最高，在人工唾液中储存的样品硬度值最低。在观察时间间隔内，无论存储介质如何，TC 的精油释放和硬度值的均值和累积值在研究组内和组间均存在统计学显著差异。
含有负载精油的 CSNPs 的 TC 似乎是治疗义齿性口炎的一种有前途的替代治疗方法，但应进行进一步的生物学分析。
