Ding Rongrong, Qiu Yibin, Zhuang Zhanwei, Ruan Donglin, Wu Jie, Zhou Shenping, Ye Jian, Cao Lu, Hong Linjun, Xu Zheng, Zheng Enqin, Li Zicong, Wu Zhenfang, Yang Jie
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangdong, 510642, PR China; Guangdong Wens Breeding Swine Technology Co., Ltd., Guangdong, 527400, PR China.
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangdong, 510642, PR China.
Theriogenology. 2021 Oct 1;173:269-278. doi: 10.1016/j.theriogenology.2021.08.012. Epub 2021 Aug 10.
With continuous improvement of sow litter size, breeders are gradually paying more attention to the quality of litter traits that directly impact the production efficiency of pig companies, such as the rate of piglets born alive (RBA) and the rate of healthy births (RHB). The objectives of this study are to dissect the genetic basis of litter traits in pig and to identify valuable genes and genetic markers, especially pleiotropic, for pig breeding. Herein, 1140 Duroc pigs and 2046 reproduction records, 5 litter traits, including the number of healthy births (NHB), number of deformed fetuses (NDF), number of stillborn (NSB), RBA, and RHB, were used in this study. Subsequently, a genome-wide association study (GWAS) was performed for the five litter traits in the first two parities from two Duroc populations. A total of 76 significantly related SNPs and 10 potential candidate genes (CAV1, DAB2, FGF12, FHOD3, DYNC2H1, GRHL1, TCTN3, PYROXD2, MMP8, MMP13, and PGR) were detected, including 13 pleiotropic SNPs that affected more than one litter trait. Finally, the functional enrichment analysis of functional genes that were closest to these significant SNPs indicated that most of the significant pathways were associated with hormone secretion and embryo and organ development. This study advances our understanding of the genetic mechanisms of litter traits, especially the survival rate of piglets born, and provides an opportunity to increase the quality of litter using marker-assisted selection or genomic selection in pigs.
随着母猪产仔数的不断提高，育种者越来越关注直接影响养猪企业生产效率的窝产性状质量，如仔猪出生成活率（RBA）和健康出生率（RHB）。本研究的目的是剖析猪窝产性状的遗传基础，并鉴定有价值的基因和遗传标记，特别是多效性基因和标记，用于猪的育种。本研究使用了1140头杜洛克猪和2046条繁殖记录，以及5个窝产性状，包括健康出生数（NHB）、畸形胎儿数（NDF）、死胎数（NSB）、RBA和RHB。随后，对两个杜洛克群体前两胎的五个窝产性状进行了全基因组关联研究（GWAS）。共检测到76个显著相关的单核苷酸多态性（SNP）和10个潜在候选基因（CAV1、DAB2、FGF12、FHOD3、DYNC2H1、GRHL1、TCTN3、PYROXD2、MMP8、MMP13和PGR），其中包括13个影响多个窝产性状的多效性SNP。最后，对最接近这些显著SNP的功能基因进行功能富集分析表明，大多数显著通路与激素分泌以及胚胎和器官发育有关。本研究增进了我们对窝产性状遗传机制的理解，特别是仔猪出生成活率，并为利用标记辅助选择或基因组选择提高猪的窝产质量提供了机会。
