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Production of a Promising Biosynthetic Self-Assembled Nanoconjugate Vaccine against Klebsiella Pneumoniae Serotype O2 in a General Escherichia Coli Host.在普通大肠杆菌宿主中生产针对肺炎克雷伯菌 O2 血清型的有前途的生物合成自组装纳米缀合物疫苗。
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Biosynthesis of Self-Assembled Proteinaceous Nanoparticles for Vaccination.自组装蛋白纳米颗粒的生物合成及其在疫苗接种中的应用。
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Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells.用于靶向抗原呈递细胞的纳米颗粒上的DNA展示
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Co-delivery of Dual Toll-Like Receptor Agonists and Antigen in Poly(Lactic-Co-Glycolic) Acid/Polyethylenimine Cationic Hybrid Nanoparticles Promote Efficient Immune Responses.聚（乳酸-共-乙醇酸）/聚乙烯亚胺阳离子杂化纳米颗粒中双重Toll样受体激动剂与抗原的共递送促进高效免疫反应
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Silica Nanoparticle as a Lymph Node Targeting Platform for Vaccine Delivery.硅纳米颗粒作为一种用于疫苗投递的淋巴结靶向平台。
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Dual Plug-and-Display Synthetic Assembly Using Orthogonal Reactive Proteins for Twin Antigen Immunization.使用正交反应性蛋白进行双抗原免疫的双插即用合成组装。
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Effects of gold nanoparticle-based vaccine size on lymph node delivery and cytotoxic T-lymphocyte responses.基于金纳米颗粒的疫苗粒径对淋巴结传递和细胞毒性 T 淋巴细胞反应的影响。
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用于抗感染疫苗接种的纳米级缀合疫苗的正交模块化生物合成。

Orthogonal modular biosynthesis of nanoscale conjugate vaccines for vaccination against infection.

作者信息

Li Xin, Pan Chao, Sun Peng, Peng Zhehui, Feng Erling, Wu Jun, Wang Hengliang, Zhu Li

机构信息

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, 100071 China.

出版信息

Nano Res. 2022;15(2):1645-1653. doi: 10.1007/s12274-021-3713-4. Epub 2021 Aug 12.

DOI:10.1007/s12274-021-3713-4
PMID:34405037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8359766/
Abstract

UNLABELLED

Conjugate vaccines represent one of the most effective means for controlling the occurrence of bacterial diseases. Although nanotechnology has been greatly applied in the field of vaccines, it is seldom used for conjugate vaccine research because it is very difficult to connect polysaccharides and nanocarriers. In this work, an orthogonal and modular biosynthesis method was used to produce nanoconjugate vaccines using the SpyTag/SpyCatcher system. When SpyTag/SpyCatcher system is combined with protein glycosylation technology, bacterial O-polysaccharide obtained from 2a can be conjugated onto the surfaces of different virus-like particles (VLPs) in a biocompatible and controlled manner. After confirming the excellent lymph node targeting and humoral immune activation abilities, these nanoconjugate vaccines further induced efficient prophylactic effects against infection in a mouse model. These results demonstrated that natural polysaccharide antigens can be easily connected to VLPs to prepare highly efficient nanoconjugate vaccines. To the best of the researchers' knowledge, this is the first time VLP-based nanoconjugate vaccines are produced efficiently, and this strategy could be applied to develop various pathogenic nanoconjugate vaccines.

ELECTRONIC SUPPLEMENTARY MATERIAL

Supplementary material (Figs. S1-S9) is available in the online version of this article at 10.1007/s12274-021-3713-4.

摘要

未标记

结合疫苗是控制细菌性疾病发生的最有效手段之一。尽管纳米技术已在疫苗领域得到广泛应用，但由于多糖与纳米载体的连接非常困难，很少用于结合疫苗的研究。在这项工作中，使用了一种正交模块化生物合成方法，利用SpyTag/SpyCatcher系统生产纳米结合疫苗。当SpyTag/SpyCatcher系统与蛋白质糖基化技术相结合时，从2a获得的细菌O-多糖可以以生物相容且可控的方式连接到不同病毒样颗粒（VLP）的表面。在确认了出色的淋巴结靶向和体液免疫激活能力后，这些纳米结合疫苗在小鼠模型中进一步诱导了针对感染的有效预防作用。这些结果表明，天然多糖抗原可以很容易地连接到VLP上，以制备高效的纳米结合疫苗。据研究人员所知，这是首次高效生产基于VLP的纳米结合疫苗，该策略可应用于开发各种致病性纳米结合疫苗。

电子补充材料

补充材料（图S1-S9）可在本文的在线版本中获取，链接为10.1007/s12274-021-3713-4。