Department of Biology, Trinity College, Hartford, CT 06106, USA.
Instituto Gulbenkian de Ciências, Oeiras, Portugal.
J Exp Biol. 2021 Aug 15;224(16). doi: 10.1242/jeb.242253. Epub 2021 Aug 18.
Fish have particularly high levels of adult neurogenesis, and this high neurogenic capacity may contribute to behavioural plasticity. While it is known that adult-born cells can differentiate into neurons and incorporate into neural circuits, it is unclear whether they are responsive to external stimuli and are thereby capable of contributing to behavioural change. We tested whether cells born in the telencephalon of adult zebrafish are activated by social stimuli. We marked cell birth with BrdU and, 40 days later, exposed fish to brief (15 min) visual social stimuli and assayed cellular activity through immunolocalization of phospho-S6-ribosomal protein (pS6). BrdU+/pS6+ co-labelled cells were found in six brain regions, and, in four regions [dorsal (D), dorsomedial (Dm) and dorsolateral (Dl) zones of the dorsal telencephalon and pre-optic area (POA)], the number of co-labelled cells and fraction of BrdU+ cells that labelled positive for pS6 increased during social stimulation. These results are consistent with the hypothesis that adult-born neurons play a role in regulating social behaviour.
鱼类具有特别高的成年神经发生水平，这种高神经发生能力可能有助于行为可塑性。虽然已知成年产生的细胞可以分化为神经元并整合到神经回路中，但尚不清楚它们是否对外部刺激有反应，从而能够促进行为改变。我们测试了成年斑马鱼脑内产生的细胞是否对社会刺激有反应。我们用 BrdU 标记细胞出生，40 天后，将鱼暴露于短暂的（15 分钟）视觉社会刺激下，并通过磷酸化 S6-核糖体蛋白（pS6）的免疫定位来检测细胞活性。在六个脑区发现了 BrdU+/pS6+共标记细胞，在四个脑区（背侧脑的背侧（D）、背内侧（Dm）和背外侧（Dl）区和视前区（POA）），共标记细胞的数量和 BrdU+细胞中标记为 pS6 的阳性细胞的比例在社会刺激期间增加。这些结果与成年产生的神经元在调节社会行为中起作用的假设一致。
