Department of Research & Development, Beckman Coulter Life Sciences-Immunotech, 130 Avenue de Lattre de Tassigny, Marseille, 13009, France.
C2VN INSERM-INRAE, Aix-Marseille University, 27 Boulevard Jean Moulin, Marseille, 13385, France.
Future Microbiol. 2021 Sep;16:955-966. doi: 10.2217/fmb-2021-0034. Epub 2021 Aug 18.
A new one-step flow cytometry procedure has been recently demonstrated for identifying subjects with infections, but only for fresh whole blood samples. The goal of this study was to assess its applicability on frozen samples, by proposing a new method to perform the sample freezing directly and easily. Fresh blood was tested, then frozen either directly or with dimethylsulfoxide and serum. Common markers of white blood cells as well as infection-related biomarkers were tested. All percentages of leucocyte subsets and levels of infection-related biomarkers were significantly correlated between frozen and fresh samples. The direct freezing method enables an accurate assessment of common cellular sub-populations and of levels of important infectious biomarkers via flow cytometry.
最近已经提出了一种新的一步式流式细胞术程序，用于鉴定感染患者，但仅适用于新鲜的全血样本。本研究的目的是通过提出一种新的方法直接简便地进行样本冷冻来评估其在冷冻样本中的适用性。对新鲜血液进行了测试，然后直接或用二甲基亚砜和血清进行冷冻。测试了白细胞常见标志物以及与感染相关的生物标志物。白细胞亚群的所有百分比以及与感染相关的生物标志物的水平在冷冻和新鲜样本之间均具有显著相关性。直接冷冻法可通过流式细胞术准确评估常见细胞亚群以及重要感染生物标志物的水平。
