Department of Fisheries, Government of Punjab, Rajanpur, 33500, Pakistan.
Department of Zoology, Faculty of Life Sciences, Government College University Faisalabad, Faisalabad, Pakistan.
Fish Physiol Biochem. 2021 Oct;47(5):1559-1564. doi: 10.1007/s10695-021-01003-x. Epub 2021 Aug 18.
The applications of exogenous hormones in different species for the induction of oocyte production, final oocyte maturation (FOM), and spawning for their reproduction is getting more attention day by day. The current preliminary research work was carried out to induce breeding in Clarias gariepinus, commonly known as African catfish, imported from Thailand. Single doses of two hormones as Ovaprim and human menopausal gonadotropin (hMG) were used and the research work was carried out at Muzaffargarh Fish Hatchery Punjab, Pakistan. A total of twenty-four (n = 24) C. gariepinus were selected having body weight approximately 2 kg and divided into two main groups based on gender as male (n = 12) and female (n = 12). For milt collection, all males were treated with Ovaprim 0.5 mg/kg body weight (b.w.) and female fish were divided into three groups as A, B, and C with four (n = 4) fish in each group. Group A was injected with only normal saline (control group) while fish in group B and group C were treated with hMG at 0.5 mg/kg b.w. and Ovaprim 0.5 mg/kg b.w., respectively. Then, after 6 h of hormone injections until 48 h, spawned eggs, eggs' weight, fertilization rate, hatching rate, survival rate, fecundity, and deformed larvae were investigated. The results revealed that Ovaprim injection significantly (p < 0.05) modulate the reproductive parameters in group C while no breeding was induced in both control and hMG-treated groups. Hence, it could be concluded that Ovaprim has the potential to induce breeding in African catfish, while in the current study, hMG failed to induce breeding. However, trials at large scales are required to further explore the effect of different doses of both tested hormones by increasing the treated subjects particularly in Pakistani fish farms.
外源激素在不同物种中的应用越来越受到关注，这些激素可用于诱导卵子产生、卵母细胞最终成熟（FOM）和产卵，从而实现繁殖。目前，初步研究工作是在从泰国进口的非洲鲶（Clarias gariepinus）中进行的，以诱导其繁殖。研究工作在巴基斯坦旁遮普省木尔坦鱼类孵化场进行，使用了两种激素，即 Ovaprim 和人绝经期促性腺激素（hMG），共选择了 24 条体重约为 2 公斤的非洲鲶，根据性别分为两组：雄性（n=12）和雌性（n=12）。为了收集精液，所有雄性都用 Ovaprim 0.5mg/kg 体重（b.w.）处理，而雌性鱼分为三组 A、B 和 C，每组 4 条。A 组仅注射生理盐水（对照组），而 B 组和 C 组分别用 hMG 0.5mg/kg b.w.和 Ovaprim 0.5mg/kg b.w.处理。然后，在注射激素后 6 小时至 48 小时内，调查产卵、卵重、受精率、孵化率、成活率、繁殖力和畸形幼虫。结果表明，Ovaprim 注射显著（p<0.05）调节了 C 组的繁殖参数，而对照组和 hMG 处理组均未诱导繁殖。因此，可以得出结论，Ovaprim 具有诱导非洲鲶繁殖的潜力，而在本研究中，hMG 未能诱导繁殖。然而，需要在更大规模的试验中进一步探索两种测试激素的不同剂量的效果，特别是在巴基斯坦的鱼类养殖场中增加处理对象。
