Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
Chem Rev. 2022 Apr 27;122(8):7386-7414. doi: 10.1021/acs.chemrev.1c00217. Epub 2021 Aug 18.
Biological mass spectrometry (MS) encompasses a range of methods for characterizing proteins and other biomolecules. MS is uniquely powerful for the structural analysis of endogenous protein complexes, which are often heterogeneous, poorly abundant, and refractive to characterization by other methods. Here, we focus on how biological MS can contribute to the study of endogenous protein complexes, which we define as complexes expressed in the physiological host and purified intact, as opposed to reconstituted complexes assembled from heterologously expressed components. Biological MS can yield information on complex stoichiometry, heterogeneity, topology, stability, activity, modes of regulation, and even structural dynamics. We begin with a review of methods for isolating endogenous complexes. We then describe the various biological MS approaches, focusing on the type of information that each method yields. We end with future directions and challenges for these MS-based methods.
生物质谱（MS）涵盖了一系列用于鉴定蛋白质和其他生物分子的方法。MS 在分析内源性蛋白质复合物的结构方面具有独特的优势，因为这些复合物通常具有异质性、丰度低，并且难以用其他方法进行鉴定。在这里，我们重点介绍生物 MS 如何有助于研究内源性蛋白质复合物，我们将其定义为在生理宿主中表达并完整纯化的复合物，而不是由异源表达的成分重新组装而成的复合物。生物 MS 可以提供关于复合物的计量学、异质性、拓扑结构、稳定性、活性、调节方式，甚至结构动力学等方面的信息。我们首先回顾了用于分离内源性复合物的方法。然后，我们描述了各种生物 MS 方法，重点介绍了每种方法所提供的信息类型。最后，我们探讨了这些基于 MS 的方法的未来方向和挑战。
