Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
Curr Opin Chem Biol. 2018 Feb;42:25-33. doi: 10.1016/j.cbpa.2017.10.026. Epub 2017 Nov 9.
Ion-mobility mass spectrometry (IM-MS) is an approach that can provide information on the stoichiometry, composition, protein contacts and topology of protein complexes. The power of this approach lies not only in its sensitivity and speed of analysis, but also in the fact that it is a technique that can capture the repertoire of conformational states adopted by protein assemblies. Here, we describe the array of available IM-MS based tools, and demonstrate their application to the structural characterization of various protein complexes, including challenging systems as amyloid aggregates and membrane proteins. We also discuss recent studies in which IM-MS was applied towards investigations of conformational transitions and stabilization effects induced by protein interactions.
离子淌度质谱（IM-MS）是一种可以提供关于蛋白质复合物的化学计量、组成、蛋白质接触和拓扑结构信息的方法。这种方法的优势不仅在于其分析的灵敏度和速度，还在于它是一种可以捕获蛋白质组装所采用的构象状态的技术。在这里，我们描述了一系列可用的基于 IM-MS 的工具，并展示了它们在各种蛋白质复合物的结构表征中的应用，包括淀粉样聚集物和膜蛋白等具有挑战性的系统。我们还讨论了最近的一些研究，其中 IM-MS 被应用于研究蛋白质相互作用诱导的构象转变和稳定化效应。
