He Haiyan, Chen Zhuo, Chen Dongjian, Lu Xu, Huang Chao, Chen Jinliang
Department of Respiratory Medicine, The Second Affiliated Hospital of Nantong University, Nantong First People's Hospital, Nantong University.
Invasive Technology Department, The Second Affiliated Hospital of Nantong University, Nantong First People's Hospital, Nantong University.
Neuroreport. 2021 Oct 6;32(14):1216-1222. doi: 10.1097/WNR.0000000000001717.
Recently, we have identified a functional expression of farnesoid X receptor (FXR) in neurons in vitro and in vivo. However, whether the FXR is expressed in astrocytes remains unclear. In the present study, we addressed this issue by using an array of experimental methods such as immunofluorescence and western blot. Results showed that the FXR mRNA and protein were expressed in mouse brain primary cultured astrocytes. In mouse primary cultured astrocytes in vitro the FXR was predominantly localized in the nucleus with an obvious punctuate distribution property. Unlike its expressional characteristic in cultured astrocytes, the FXR was not detected in astrocytes in the mouse hippocampus and prefrontal cortex, suggesting that the FXR is not expressed in astrocytes at conditions in vivo. Functional studies in vitro showed that activation of the FXR in primary cultured astrocytes by chenodeoxycholic acid or GW4064 induced a marked increase in expression levels of small heterodimer partner mRNA and protein. Taken together, these findings show a differential expression of FXR in astrocytes at conditions in vitro but not in vivo, and in mouse primary cultured astrocytes the FXR can be activated by its ligands.
最近，我们已经在体外和体内神经元中鉴定出法尼酯X受体（FXR）的功能性表达。然而，FXR是否在星形胶质细胞中表达仍不清楚。在本研究中，我们通过使用一系列实验方法，如免疫荧光和蛋白质印迹，来解决这个问题。结果显示，FXR mRNA和蛋白质在小鼠脑原代培养星形胶质细胞中表达。在体外小鼠原代培养星形胶质细胞中，FXR主要定位于细胞核，具有明显的点状分布特性。与其在培养星形胶质细胞中的表达特征不同，在小鼠海马体和前额叶皮质的星形胶质细胞中未检测到FXR，这表明在体内条件下FXR不在星形胶质细胞中表达。体外功能研究表明，鹅去氧胆酸或GW4064激活原代培养星形胶质细胞中的FXR会导致小异源二聚体伴侣mRNA和蛋白质的表达水平显著增加。综上所述，这些发现表明FXR在体外而非体内条件下在星形胶质细胞中存在差异表达，并且在小鼠原代培养星形胶质细胞中FXR可被其配体激活。
