Kiser Jennifer N, Neibergs Holly L
Department of Animal Sciences, Washington State University, Pullman, WA, United States.
Front Vet Sci. 2021 Aug 2;8:679074. doi: 10.3389/fvets.2021.679074. eCollection 2021.
Bovine coronavirus (BCoV) is associated with respiratory and enteric infections in both dairy and beef cattle worldwide. It is also one of a complex of pathogens associated with bovine respiratory disease (BRD), which affects millions of cattle annually. The objectives of this study were to identify loci and heritability estimates associated with BCoV infection and BRD in dairy calves and feedlot cattle. Dairy calves from California ( = 1,938) and New Mexico ( = 647) and feedlot cattle from Colorado ( = 915) and Washington ( = 934) were tested for the presence of BCoV when classified as BRD cases or controls following the McGuirk scoring system. Two comparisons associated with BCoV were investigated: (1) cattle positive for BCoV (BCoV) were compared to cattle negative for BCoV (BCoV) and (2) cattle positive for BCoV and affected with BRD (BCoVBRD) were compared to cattle negative for BCoV and BRD (BCoVBRD). The Illumina BovineHD BeadChip was used for genotyping, and genome-wide association analyses (GWAA) were performed using EMMAX (efficient mixed-model association eXpedited). The GWAA for BCoV identified 51 loci ( < 1 × 10; 24 feedlot, 16 dairy, 11 combined) associated with infection with BCoV. Three loci were associated with BCoV across populations. Heritability estimates for BCoV were 0.01 for dairy, 0.11 for feedlot cattle, and 0.03 for the combined population. For BCoVBRD, 80 loci ( < 1 × 10; 26 feedlot, 25 dairy, 29 combined) were associated including 14 loci across populations. Heritability estimates for BCoVBRD were 0.003 for dairy, 0.44 for feedlot cattle, and 0.07 for the combined population. Several positional candidate genes associated with BCoV and BRD in this study have been associated with other coronaviruses and respiratory infections in humans and mice. These results suggest that selection may reduce susceptibility to BCoV infection and BRD in cattle.
牛冠状病毒（BCoV）与全球范围内奶牛和肉牛的呼吸道及肠道感染有关。它也是与牛呼吸道疾病（BRD）相关的一组病原体之一，BRD每年影响数百万头牛。本研究的目的是确定与奶牛犊牛和育肥牛场中BCoV感染及BRD相关的基因座和遗传力估计值。根据麦吉尔克评分系统，将来自加利福尼亚州（n = 1938）和新墨西哥州（n = 647）的奶牛犊牛以及来自科罗拉多州（n = 915）和华盛顿州（n = 934）的育肥牛场牛在被分类为BRD病例或对照时检测BCoV的存在情况。研究了与BCoV相关的两项比较：（1）BCoV阳性的牛（BCoV⁺）与BCoV阴性的牛（BCoV⁻）进行比较；（2）BCoV阳性且患有BRD的牛（BCoV⁺BRD）与BCoV和BRD均为阴性的牛（BCoV⁻BRD⁻）进行比较。使用Illumina BovineHD BeadChip进行基因分型，并使用EMMAX（高效混合模型关联加速版）进行全基因组关联分析（GWAA）。BCoV的GWAA确定了51个与BCoV感染相关的基因座（P < 1×10⁻⁵；24个来自育肥牛场，16个来自奶牛场，11个为综合数据）。三个基因座在不同群体中均与BCoV相关。奶牛群体中BCoV的遗传力估计值为0.01，育肥牛场牛为0.11，综合群体为0.03。对于BCoV⁺BRD，有80个基因座（P < 1×10⁻⁵；26个来自育肥牛场，25个来自奶牛场，29个为综合数据）与之相关，其中包括14个在不同群体中均相关的基因座。奶牛群体中BCoV⁺BRD的遗传力估计值为0.003，育肥牛场牛为0.44，综合群体为0.07。本研究中几个与BCoV和BRD相关的定位候选基因已被证明与人类和小鼠中的其他冠状病毒及呼吸道感染有关。这些结果表明，选择育种可能会降低牛对BCoV感染和BRD的易感性。
