Liu Kai, Feng Xiao-Yu, Ma Heng-Jia, Xie Nan
Institute of Fishery Science, Hangzhou Academy of Agricultural Sciences, Hangzhou, China.
Mitochondrial DNA B Resour. 2021 Aug 10;6(9):2601-2604. doi: 10.1080/23802359.2021.1961627. eCollection 2021.
This study determined the mitochondrial genome (mitogenome) of from China's Qiantang River and analyzed its phylogenetic history in the Subfamily Cultrinae. Next-generation sequencing was used to obtain the mitogenome of , GenBank Accession Number MZ032228. The mitochondrial genome length of from China's Qiantang River is 16,622 bp. The genome contains 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two central noncoding regions (the control region and the origin of light strand replication). Based on BLAST comparisons, the sequence identity of MZ032228 from China's Qiantang River was 99.84% to that of MG783573 from China's Nei River, 99.75% to AP009060 from Russia's Black River. The phylogenetic analysis is consistent with BLAST comparisons in confirming that MG783573 and MZ032228 show a high genetic similarity. This study also confirms mitochondrial DNA heteroplasmy in for the first time and documents 35 heterogeneous loci that were detected.
本研究测定了中国钱塘江[鱼名未给出]的线粒体基因组（线粒体基因组），并分析了其在雅罗鱼亚科中的系统发育历史。采用二代测序技术获得了[鱼名未给出]的线粒体基因组，GenBank登录号为MZ032228。中国钱塘江[鱼名未给出]的线粒体基因组长度为16,622 bp。该基因组包含13个蛋白质编码基因、22个转运RNA、两个核糖体RNA以及两个中央非编码区（控制区和轻链复制起点）。基于BLAST比较，中国钱塘江MZ032228与中国内河流域MG783573的序列同一性为99.84%，与俄罗斯黑河AP009060的序列同一性为99.75%。系统发育分析与BLAST比较结果一致，证实MG783573和MZ032228具有高度的遗传相似性。本研究还首次证实了[鱼名未给出]线粒体DNA异质性，并记录了检测到的35个异质位点。
