Aksöz N
H. U. Fen Fakültesi Biyoloji Bölümü, Beytepe/Ankara.
Mikrobiyol Bul. 1987 Jan;21(1):34-41.
In this study, culture supernatant of Bacillus sp. has been used as crude enzyme source. It has been determined that 1% urea is the optimal substrate concentration for maximal urease activity. On the other hand, Bacillus sp. urease showed its maximal activity at pH:7.0-8.0, 30 degrees C. 50 minutes of incubation has been found to be the most suitable incubation period. The activity of the enzyme showed a 36% decrease when stored at +4 degrees C for a month.
在本研究中，芽孢杆菌属的培养上清液已被用作粗酶源。已确定1%尿素是实现最大脲酶活性的最佳底物浓度。另一方面，芽孢杆菌属脲酶在pH值为7.0 - 8.0、30摄氏度时表现出最大活性。已发现50分钟的孵育时间是最合适的孵育期。该酶在4摄氏度下储存一个月后活性降低了36%。
