Chen Y, Long J, Liao L, Zhang Y, Yang J
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041.
Wei Sheng Wu Xue Bao. 2000 Feb;40(1):62-8.
Bacillus sp. M50 produced beta-mannanase efficiently in the medium composed of 4% konjac powder, 1.0% (NH4)2SO4 and 0.35% Na2CO3. Fermentation of beta-mannanase by Bacillus sp. M50 has been carried out in 100 L tank. The temperature, ventitation quantity and agitation speed were 30-32 degrees C, 1:0.75 vvm and 220 r/min respectively. the fermentation cycle was 28 h and the highest beta-mannanase activity was 330 u/mL. The optimal temperature and pH for beta-mannanase reaction were 50 degrees C and 6.0 respectively. It was stable at pH 4.0-7.0 and below 50 degrees C. The activity of the enzyme was strongly inhibited by Fe3+, Al3+, EDTA, Hg2+ and slightly stimulated by Ba2+, Mn2+. Treatment of ramie-fibers with crude enzyme preparations from Bacillus sp. M50 showed a good degradation for hemicellulose of ramie-fibers residues.
芽孢杆菌属M50在由4%魔芋粉、1.0%硫酸铵和0.35%碳酸钠组成的培养基中能高效产生β-甘露聚糖酶。芽孢杆菌属M50对β-甘露聚糖酶的发酵在100 L发酵罐中进行。温度、通气量和搅拌速度分别为30 - 32℃、1:0.75 vvm和220 r/min。发酵周期为28 h，β-甘露聚糖酶的最高活性为330 u/mL。β-甘露聚糖酶反应的最适温度和pH分别为50℃和6.0。它在pH 4.0 - 7.0和低于50℃时稳定。该酶的活性受到Fe3+、Al3+、EDTA、Hg2+的强烈抑制，受到Ba2+、Mn2+的轻微刺激。用芽孢杆菌属M50的粗酶制剂处理苎麻纤维，结果表明对苎麻纤维残渣中的半纤维素有良好的降解效果。
