Maeda S, Shimada K, Takagi Y
Gene. 1978 Feb;3(1):1-7. doi: 10.1016/0378-1119(78)90002-1.
Insertion of the ampicillin transposon (Tn3) into ColE1 DNAs causes various mutations in the plasmids. Escherichia coli K-12 cells carrying one of these mutants showed novel properties; they were sensitive to colicin E1 and were able to produce active colicin E1. The site and the orientation of Tn3 insertion in this mutant ColE1 DNA were determined by heteroduplex analysis and by enzymatic digestion with restriction endonucleases. The potential usefulness of this mutant ColE1 DNA as a cloning vehicle is discussed.
将氨苄青霉素转座子（Tn3）插入ColE1 DNA会导致质粒发生各种突变。携带这些突变体之一的大肠杆菌K-12细胞表现出新颖的特性；它们对大肠杆菌素E1敏感，并且能够产生活性大肠杆菌素E1。通过异源双链分析和限制性内切酶酶切确定了该突变体ColE1 DNA中Tn3插入的位点和方向。讨论了这种突变体ColE1 DNA作为克隆载体的潜在用途。
