骨关节结核的诊断：多靶标环介导等温扩增检测法与多重 PCR 检测法的比较。
Diagnosis of osteoarticular tuberculosis: multi-targeted loop-mediated isothermal amplification assay versus multiplex PCR.
机构信息
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, 124001, India.
Department of Orthopaedics, University of Health Sciences, Rohtak, 124001, India.
出版信息
Future Microbiol. 2021 Sep;16:935-948. doi: 10.2217/fmb-2021-0030. Epub 2021 Aug 20.
Diagnosis of osteoarticular tuberculosis (OATB) is quite challenging and there is an urgent need to design a prompt and precise diagnostic test. We developed a multi-targeted loop-mediated isothermal amplification (LAMP) assay using (Rv1980c) and (Rv0934) targets for the detection of in OATB patients. The sensitivities of 100 and 82.4% were obtained in confirmed (n = 10) and suspected (n = 57) OATB cases, respectively by multi-targeted LAMP with a specificity of 96.9% (n = 33). Moreover, the sensitivities attained by multi-targeted LAMP in total OATB cases were significantly higher (p < 0.05-0.01) than multiplex PCR ( + ) and GeneXpert assay. Our LAMP is simple, reliable and cost-effective method, which may develop into an attractive diagnostic kit for early detection of OATB cases.
骨关节炎结核（OATB）的诊断极具挑战性，因此迫切需要设计一种快速而准确的诊断测试。我们使用（Rv1980c）和（Rv0934）靶标开发了一种多靶向环介导等温扩增（LAMP）检测方法，用于检测 OATB 患者中的。多靶向 LAMP 的灵敏度分别为 100%和 82.4%，在确诊（n=10）和疑似（n=57）OATB 病例中，特异性为 96.9%（n=33）。此外，多靶向 LAMP 在所有 OATB 病例中的灵敏度均明显高于多重 PCR（+）和 GeneXpert 检测（p<0.05-0.01）。我们的 LAMP 是一种简单、可靠且具有成本效益的方法，可能会发展成为一种有吸引力的 OATB 早期检测诊断试剂盒。
Zotero 插件