Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug, Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug, Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Neuroscience. 2021 Oct 1;473:130-141. doi: 10.1016/j.neuroscience.2021.08.006. Epub 2021 Aug 17.
Alpha-asarone, a major active component isolated from Acorus gramineus, can affect brain functions and behaviors by multiple mechanisms. However, the effect of alpha-asarone on cerebral ischemia-reperfusion (CIR) stroke has not been reported. The present study aimed to investigate the neuroprotective effect of alpha-asarone and the involved mechanisms against CIR stroke. Rats were subjected to middle cerebral occlusion (MCAO) for 2 h. Then the drug or drug-free vehicle was intravenously injected to corresponding groups. After reperfusion for 24 h, the infarct volume was evaluated by Triphenyl Tetrazolium Chloride (TTC) staining. The neurofunctional recovery and post-stroke epilepsy were evaluated. Nissl and Hematoxylin-Eosin (H&E) staining were used for histological observation. We investigated the protective mechanism of alpha-asarone against the stroke. The results showed that alpha-asarone exhibited a desirable neuroprotective effect, manifested as reducing infarct volume and post-stroke epilepsy and improving neurological function. Histological and flow cytometry analysis revealed that alpha-asarone treatment alleviated cell injury and apoptosis in vivo and in vitro. Furthermore, alpha-asarone decreased GFAP, Iba-1, and LC3II/LC3I expression and increased the expression of p62. These results suggested that alpha-asarone attenuated the CIR stroke injury via ameliorating glial activation and autophagy.
α-细辛脑是菖蒲中提取的一种主要活性成分，可通过多种机制影响脑功能和行为。然而，α-细辛脑对脑缺血再灌注（CIR）卒中的影响尚未见报道。本研究旨在探讨α-细辛脑对 CIR 卒中的神经保护作用及其机制。大鼠大脑中动脉闭塞（MCAO）2 h 后，静脉注射药物或无药物载体。再灌注 24 h 后，用氯化三苯基四氮唑（TTC）染色评估梗死体积。评估神经功能恢复和卒中后癫痫。尼氏染色和苏木精-伊红（H&E）染色用于组织学观察。我们研究了α-细辛脑对中风的保护机制。结果表明，α-细辛脑具有良好的神经保护作用，可减少梗死体积和卒中后癫痫发作，改善神经功能。组织学和流式细胞术分析显示，α-细辛脑治疗可减轻体内和体外的细胞损伤和凋亡。此外，α-细辛脑降低了 GFAP、Iba-1 和 LC3II/LC3I 的表达，增加了 p62 的表达。这些结果表明，α-细辛脑通过改善神经胶质细胞激活和自噬来减轻 CIR 卒中损伤。
