College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China.
College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China.
Carbohydr Polym. 2021 Nov 15;272:118498. doi: 10.1016/j.carbpol.2021.118498. Epub 2021 Jul 29.
Construction of monolithic cryogels that can efficiently adsorb proteins is of great significance in biotechnological and pharmaceutical industries. Herein, a novel approach is presented to fabricate microfibrillated cellulose (MFC)/sodium alginate (SA) cryogels by using freezing-induced oxa-Michael reaction at -12 °C. Thanks to the controllable reactiveness of divinyl sulfone (DVS), cryo-concentrated pH increase activates the oxa-Michael reaction between DVS and hydroxyl groups of MFCs and SAs. The obtained composite cryogel exhibits outstanding underwater shape recovery and excellent fatigue resistance. Moreover, the MFC/SAs reveal a high lysozyme adsorption capacity of 294.12 mg/g, surpassing most of absorbent materials previously reported. Furthermore, the cryogel-packed column can purify lysozyme continuously from chicken egg white, highlighting its outstanding practical application performance. Reuse experiments indicated that over 90% of lysozyme extraction capacity was retained after 6 cycles. This work provides a new avenue to design and develop next-generation chromatographic media of natural polysaccharide-based cryogel for protein purification.
制备能够高效吸附蛋白质的整体式冷冻凝胶在生物技术和制药行业具有重要意义。在此，我们提出了一种新方法，即在-12°C 下通过冷冻诱导的氧杂迈克尔反应来制备微纤化纤维素（MFC）/海藻酸钠（SA）冷冻凝胶。由于二乙烯砜（DVS）的可控反应性，冷冻浓缩 pH 值的增加会激活 DVS 与 MFC 和 SA 上的羟基之间的氧杂迈克尔反应。所得到的复合冷冻凝胶具有出色的水下形状恢复能力和优异的耐疲劳性。此外，MFC/SA 对溶菌酶的吸附容量高达 294.12mg/g，超过了之前报道的大多数吸附材料。此外，该冷冻凝胶填充柱可以从鸡蛋白中连续纯化溶菌酶，突出了其出色的实际应用性能。重复使用实验表明，在 6 个循环后，仍保留超过 90%的溶菌酶提取能力。这项工作为设计和开发用于蛋白质纯化的新一代基于天然多糖的色谱介质提供了新途径。
