Wick G, Brunner H, Penner E, Timpl R
Int Arch Allergy Appl Immunol. 1978;56(4):316-24. doi: 10.1159/000232037.
Frozen, unfixed sections of human liver biopsies from patients with acute, subchronic, and chronic hepatitis or fibrotic liver disease were studied in indirect immunofluorescence with specific antisera to type I and type III procollagen. In early stages of both hepatitis and fibrotic liver disease, intralobular type III collagen synthesis is increased. Maximum values are reached years after the onset of disease. Intralobular procollagen I content is not increased in the acute stage, but rises only later. An increase of procollagen I seems to herald irreversible liver changes. This approach allows for exact localization and semiquantitative analysis of the synthesis of type I and type III collagen, and adds a new parameter to the diagnostic approaches in liver diseases.
采用针对I型和III型前胶原的特异性抗血清，通过间接免疫荧光法对患有急性、亚慢性和慢性肝炎或肝纤维化疾病患者的人体肝脏活检冷冻、未固定切片进行了研究。在肝炎和肝纤维化疾病的早期阶段，小叶内III型胶原合成增加。在疾病发作数年之后达到最大值。急性期小叶内前胶原I含量并未增加，只是在之后才升高。前胶原I增加似乎预示着肝脏发生不可逆变化。这种方法能够对I型和III型胶原的合成进行精确定位和半定量分析，并为肝病诊断方法增添了一个新参数。
