禽腺病毒血清型 4（FAdV-4）诱导鸡的细胞途径产生干扰素和抗原呈递分子（MHCI/II）。
The fowl adenovirus serotype 4 (FAdV-4) induce cellular pathway in chickens to produce interferon and antigen-presented molecules (MHCI/II).
机构信息
Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, 230036, PR China.
Anhui Academy of Agricultural Sciences, Hefei, Anhui, 230036, PR China.
出版信息
Poult Sci. 2021 Oct;100(10):101406. doi: 10.1016/j.psj.2021.101406. Epub 2021 Jul 24.
FAdV-4 is the major strain of adenovirus that responsible for hydro-pericardial syndrome (HPS) in poultry. In this study, the virus's specific gene fragments were isolated from clinically suspected cases and amplified by PCR. Finally, after a viral infection to investigate the immune response of the host, the gene expression of MHC (major histo-compatible) molecules (MHCIα, MHCIIβ), Ii (Invariant Chain) gene, inflammatory cytokines (IFN-β, IFN-γ, and IL-1β), and transcription factors (MDA5, STING, IRF7, and NF-kB) were detected by real-time PCR (fluorescence technology). The results of sequence comparison showed that the clinically isolated virus was 100% homologous to a virulent strain of avian adenovirus group C serotype 4 (FAdV-4), which were named AH-FAdV-4. The TCID and pathogenicity of the virus were determined that was 10/0.1 mL with a mortality rate of 100% in chickens and 0% in ducks. Furthermore, results showed that the expression level of MHCIα, MHCIIβ, and Ii genes in chicken embryo kidney cells significantly (P < 0.01) upregulated (increased) after infection, which was 43, 5.2, and 2.5 times higher than the control group. With the addition of PDTC, an inhibitor of NF-kB, then the expression level of MHCIα, MHCIIβ, and Ii was decreased significantly (P < 0.01) than the control group. The transcription levels of these genes were decreased 0.64, 0.27, and 0.26 respectively. Simultaneously, the expression levels of IFN-β, IFN-γ, and IL-1β were also significantly (P < 0.01) up-regulated (increased) 7.8, 22.7, and 5 times higher than the control group. It was found that up-regulation of STING and NF-κB pathways are directly involved in the regulation of inflammatory cytokines (IFN-β, IFN-γ, and IL-1β), MHC molecules (MHCIα, MHCIIβ), and Ii gene. The results also showed that the gene regulation pathways consecutively increased the expression levels of MDA5, STING, IRF7, and NF-kB. It is conducted that the expression levels of cytokines, MHC molecules, and li gene were increased by STING and NF-kB pathways.
FAdV-4 是导致家禽包涵体肝炎（HPS）的主要腺病毒株。在本研究中，通过 PCR 从临床疑似病例中分离出病毒的特定基因片段。最后，通过病毒感染来研究宿主的免疫反应，通过实时 PCR（荧光技术）检测 MHC（主要组织相容性）分子（MHCIα、MHCIIβ）、Ii（不变链）基因、炎性细胞因子（IFN-β、IFN-γ 和 IL-1β）和转录因子（MDA5、STING、IRF7 和 NF-kB）的基因表达。序列比较结果表明，从临床分离的病毒与禽腺病毒 C 群 4 型（FAdV-4）强毒 100%同源，命名为 AH-FAdV-4。病毒的 TCID 和致病性测定为 10/0.1 mL，鸡死亡率为 100%，鸭死亡率为 0%。此外，结果表明，感染后鸡胚肾细胞中 MHCIα、MHCIIβ 和 Ii 基因的表达水平显著（P<0.01）上调，分别比对照组高 43、5.2 和 2.5 倍。加入 NF-kB 抑制剂 PDTC 后，MHCIα、MHCIIβ 和 Ii 的表达水平显著（P<0.01）低于对照组，这些基因的转录水平分别下降 0.64、0.27 和 0.26。同时，IFN-β、IFN-γ 和 IL-1β 的表达水平也显著（P<0.01）上调，分别比对照组高 7.8、22.7 和 5 倍。发现 STING 和 NF-κB 途径的上调直接参与调节炎性细胞因子（IFN-β、IFN-γ 和 IL-1β）、MHC 分子（MHCIα、MHCIIβ）和 Ii 基因。结果还表明，基因调控途径依次增加 MDA5、STING、IRF7 和 NF-kB 的表达水平。研究表明，通过 STING 和 NF-kB 途径可增加细胞因子、MHC 分子和 li 基因的表达水平。
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