Schardl C L, Byrd A D, Benzion G, Altschuler M A, Hildebrand D F, Hunt A G
Department of Plant Pathology, University of Kentucky, Lexington 40546-0091.
Gene. 1987;61(1):1-11. doi: 10.1016/0378-1119(87)90359-3.
We have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. These vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35S promoter or the pea rbcS-E9 promoter, a multiple cloning site derived from M13um20, and the rbcS-E9 polyadenylation site. These cassettes have been incorporated into pBR322-based or RK2-based replicons to facilitate direct DNA uptake or Agrobacterium tumefaciens-mediated gene transfer. Their application for the expression of a bacterial gene is described.
我们构建了一系列载体，以实现外源基因在植物中的组成型表达或光调控表达。这些载体携带的表达盒由花椰菜花叶病毒35S启动子或豌豆rbcS-E9启动子、源自M13um20的多克隆位点以及rbcS-E9聚腺苷酸化位点组成。这些表达盒已被整合到基于pBR322或基于RK2的复制子中，以促进直接DNA摄取或根癌农杆菌介导的基因转移。文中描述了它们在细菌基因表达中的应用。
