Gil-Solsona R, Rodriguez-Mozaz S, Diaz-Cruz M S, Sunyer-Caldú A, Luarte T, Höfer J, Galbán-Malagón C, Gago-Ferrero P
Catalan Institute for Water Research, Girona, Spain.
Institute of Environmental Assessment and Water Research (IDAEA) Severo Ochoa Excellence Center, Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, E-08034 Barcelona, Spain.
MethodsX. 2021 Jan 5;8:101193. doi: 10.1016/j.mex.2020.101193. eCollection 2021.
This work describes a robust and powerful method for wide-scope target and non-target analysis of xenobiotics in biota samples based on bead beating tissuelyser extraction, solid phase extraction (SPE) clean-up and further detection by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS). Unlike target methodologies, non-target methods usually aim at determining a wide range of still unknown substances with different physicochemical properties. Therefore, losses during the extraction process were minimised. Apart from that, the reduction of possible interferences showed to be necessary to expand the number of compounds that can be detected. This was achieved with an additional SPE clean-up step carried out with mixed-bed multi-layered cartridges. The method was validated with a set of 27 compounds covering a wide range of physicochemical properties, and further applied to the analysis of krill and fish samples.•The bead beating extraction was efficient for a wide range of organic pollutants in small quantities of biota samples.•Multi-layered solid phase extraction clean-up yield a wide xenobiotics coverage reducing matrix effects.•Method validation with 27 compounds led to a suitable method for non-target analysis of organic pollutants in biota.
这项工作描述了一种强大且有效的方法，用于基于珠磨组织匀浆器提取、固相萃取（SPE）净化以及液相色谱-高分辨率质谱联用（LC-HRMS）进一步检测，对生物群样本中的外源性物质进行广泛的目标和非目标分析。与目标分析方法不同，非目标分析方法通常旨在确定范围广泛的、具有不同物理化学性质的未知物质。因此，提取过程中的损失被降至最低。除此之外，减少可能的干扰对于扩大可检测化合物的数量而言显得很有必要。这是通过使用混合床多层柱进行额外的SPE净化步骤来实现的。该方法用一组涵盖广泛物理化学性质的27种化合物进行了验证，并进一步应用于磷虾和鱼类样本的分析。•珠磨提取对于少量生物群样本中的多种有机污染物是有效的。•多层固相萃取净化可实现广泛的外源性物质覆盖，减少基质效应。•用27种化合物进行方法验证，得到了一种适用于生物群中有机污染物非目标分析的方法。
