School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, China.
Physiol Plant. 2021 Dec;173(4):1956-1966. doi: 10.1111/ppl.13539. Epub 2021 Sep 7.
Cyclocarya paliurus has traditionally been used as medicine and functional food. This study aims at investigating the flavonoid accumulation in C. paliurus dependent on phosphate (Pi) availability and its potential association with internal carbon partitioning. One-year-old seedlings of C. paliurus were planted in four different Pi levels. Low Pi resulted in low phosphorus content within plants, while the nitrogen content increased. Further analysis revealed that the surplus carbon pool was greater and was allocated to N-metabolism and carbohydrate synthesis under low Pi conditions, as shown by the higher levels of free amino acids, starch, and soluble sugars. Low Pi availability also induced higher enzymatic activities of shikimate dehydrogenase (SDH) and flavonoid 3-hydoxylase (FHT), and higher flavonoid accumulation in leaves. Our results indicated that the surplus carbon induced by low-Pi levels can increase flavonoid synthesis in seedlings of C. paliurus. In addition, growth and biomass accumulation were increased by the elevated Pi levels. As a result, the highest flavonoid yield per plant was obtained under relative low Pi conditions. This study can provide the basis for developing new agricultural practices to maintain high yield while still keeping the quality of medicinal plants and crops.
青钱柳传统上被用作药物和功能性食品。本研究旨在调查磷（Pi）供应依赖性青钱柳中类黄酮的积累及其与内部碳分配的潜在关联。将 1 年生青钱柳幼苗种植在 4 种不同的 Pi 水平中。低 Pi 导致植物内磷含量低，而氮含量增加。进一步分析表明，低 Pi 条件下，剩余碳库更大，并分配给 N 代谢和碳水化合物合成，表现为游离氨基酸、淀粉和可溶性糖水平更高。低 Pi 供应还诱导了更高的莽草酸脱氢酶（SDH）和类黄酮 3-羟化酶（FHT）的酶活性，以及叶片中类黄酮的积累增加。我们的结果表明，低 Pi 水平诱导的剩余碳可以增加青钱柳幼苗中类黄酮的合成。此外，Pi 水平的升高增加了生长和生物量的积累。因此，在相对低 Pi 条件下获得了每株植物最高的类黄酮产量。本研究可为开发新的农业实践提供依据，在保持药用植物和作物质量的同时保持高产量。
