Division of Life Sciences and Medicine, School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Hefei 230026, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Biosensors (Basel). 2021 Aug 20;11(8):286. doi: 10.3390/bios11080286.
Traditional in vitro anticancer drug sensitivity testing at the population level suffers from lengthy procedures and high false positive rates. To overcome these defects, we built a confocal Raman microscopy sensing system and proposed a single-cell approach via Raman-deuterium isotope probing (Raman-DIP) as a rapid and reliable in vitro drug efficacy evaluation method. Raman-DIP detected the incorporation of deuterium into the cell, which correlated with the metabolic activity of the cell. The human non-small cell lung cancer cell line HCC827 and human breast cancer cell line MCF-7 were tested against eight different anticancer drugs. The metabolic activity of cancer cells could be detected as early as 12 h, independent of cell growth. Incubation of cells in 30% heavy water (DO) did not show any negative effect on cell viability. Compared with traditional methods, Raman-DIP could accurately determine the drug effect, meanwhile, it could reduce the testing period from 72-144 h to 48 h. Moreover, the heterogeneity of cells responding to anticancer drugs was observed at the single-cell level. This proof-of-concept study demonstrated the potential of Raman-DIP to be a reliable tool for cancer drug discovery and drug susceptibility testing.
传统的群体水平抗癌药物药敏试验存在过程冗长和假阳性率高的问题。为了克服这些缺陷，我们构建了一个共聚焦拉曼显微镜传感系统，并提出了一种通过拉曼氘同位素探测（Raman-DIP）的单细胞方法，作为一种快速可靠的体外药物疗效评估方法。拉曼氘同位素探测检测氘掺入细胞的情况，这与细胞的代谢活性相关。我们用八种不同的抗癌药物对人非小细胞肺癌细胞系 HCC827 和人乳腺癌细胞系 MCF-7 进行了测试。早在 12 小时，就可以检测到癌细胞的代谢活性，而与细胞生长无关。细胞在 30%重水中（DO）孵育不会对细胞活力产生任何负面影响。与传统方法相比，Raman-DIP 不仅可以更准确地确定药物的效果，还可以将测试周期从 72-144 小时缩短至 48 小时。此外，还在单细胞水平观察到了细胞对抗癌药物反应的异质性。这项概念验证研究表明，Raman-DIP 有可能成为癌症药物发现和药敏试验的可靠工具。
