Otero-Blanca Adriana, Pérez-Llano Yordanis, Reboledo-Blanco Guillermo, Lira-Ruan Verónica, Padilla-Chacon Daniel, Folch-Mallol Jorge Luis, Sánchez-Carbente María Del Rayo, Ponce De León Inés, Batista-García Ramón Alberto
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico.
Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay.
J Fungi (Basel). 2021 Aug 22;7(8):677. doi: 10.3390/jof7080677.
Anthracnose caused by the hemibiotroph fungus is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of has been studied in a number of angiosperm crops. To better understand the evolution of the plant response to pathogens, the study of this complex interaction has been extended to bryophytes. The model moss Hedw. B&S (former ) is sensitive to known bacterial and fungal phytopathogens, including , which cause infection and cell death. responses to these microorganisms resemble that of the angiosperms. However, the molecular events during the interaction of and have not been explored. In this work, we present a comprehensive approach using microscopy, phenomics and RNA-seq analysis to explore the defense response of to . Microscopy analysis showed that appressoria are already formed at 24 h after inoculation (hai) and tissue colonization and cell death occur at 24 hai and is massive at 48 hai. Consequently, the phenomics analysis showed progressing browning of moss tissues and impaired photosynthesis from 24 to 48 hai. The transcriptomic analysis revealed that more than 1200 genes were differentially expressed in response to infection. The analysis of differentially expressed gene function showed that the infection led to a transcription reprogramming in that upregulated the genes related to pathogen recognition, secondary metabolism, cell wall reinforcement and regulation of gene expression. In accordance with the observed phenomics results, some photosynthesis and chloroplast-related genes were repressed, indicating that, under attack, changes its transcription from primary metabolism to defend itself from the pathogen.
由半活体营养型真菌引起的炭疽病是一种极具破坏性的植物病害，对植物生产力有着广泛影响。在许多被子植物作物中都对其定殖和病害发展过程进行了研究。为了更好地理解植物对病原体反应的进化，这种复杂相互作用的研究已扩展到苔藓植物。模式苔藓Hedw. B&S（原 ）对已知的细菌和真菌植物病原体敏感，包括 ，这些病原体会导致感染和细胞死亡。 对这些微生物的反应与被子植物相似。然而， 与 相互作用过程中的分子事件尚未得到探索。在这项工作中，我们采用了一种综合方法，利用显微镜、表型组学和RNA测序分析来探索 对 的防御反应。显微镜分析表明，接种后24小时（hai）附着胞已经形成，组织定殖和细胞死亡在24 hai时发生，并在48 hai时大量出现。因此，表型组学分析表明，从24到48 hai，苔藓组织逐渐褐变，光合作用受损。转录组分析显示，超过1200个 基因在对 感染的反应中差异表达。对差异表达基因功能的分析表明， 感染导致 中的转录重编程，上调了与病原体识别、次生代谢、细胞壁强化和基因表达调控相关的基因。与观察到的表型组学结果一致，一些光合作用和叶绿体相关基因被抑制，这表明在受到攻击时， 会将其转录从初级代谢转变为防御病原体。
