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烟草液泡膜内在蛋白 423（NtMLP423）正向调控干旱耐受性，依赖于 ABA 信号途径。

The Nicotiana tabacum L. major latex protein-like protein 423 (NtMLP423) positively regulates drought tolerance by ABA-dependent pathway.

机构信息

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, P.R. China.

Linyi University, Linyi, 276005, Shandong, P.R. China.

出版信息

BMC Plant Biol. 2020 Oct 16;20(1):475. doi: 10.1186/s12870-020-02690-z.

DOI:10.1186/s12870-020-02690-z
PMID:33066728
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7565365/
Abstract

BACKGROUND

Drought stress is an environmental factor that limits plant growth and reproduction. Little research has been conducted to investigate the MLP gene in tobacco. Here, NtMLP423 was isolated and identified, and its role in drought stress was studied.

RESULTS

Overexpression of NtMLP423 improved tolerance to drought stress in tobacco, as determined by physiological analyses of water loss efficiency, reactive oxygen species levels, malondialdehyde content, and levels of osmotic regulatory substances. Overexpression of NtMLP423 in transgenic plants led to greater sensitivity to abscisic acid (ABA)-mediated seed germination and ABA-induced stomatal closure. NtMLP423 also regulated drought tolerance by increasing the levels of ABA under conditions of drought stress. Our study showed that the transcription level of ABA synthetic genes also increased. Overexpression of NtMLP423 reduced membrane damage and ROS accumulation and increased the expression of stress-related genes under drought stress. We also found that NtWRKY71 regulated the transcription of NtMLP423 to improve drought tolerance.

CONCLUSIONS

Our results indicated that NtMLP423-overexpressing increased drought tolerance in tobacco via the ABA pathway.

摘要

背景

干旱胁迫是限制植物生长和繁殖的环境因素。对烟草 MLP 基因的研究较少。本研究分离并鉴定了 NtMLP423，并研究了其在干旱胁迫中的作用。

结果

通过对失水效率、活性氧水平、丙二醛含量和渗透调节物质水平的生理分析，发现过表达 NtMLP423 提高了烟草对干旱胁迫的耐受性。在转基因植物中过表达 NtMLP423 导致对脱落酸（ABA）介导的种子萌发和 ABA 诱导的气孔关闭更加敏感。NtMLP423 还通过在干旱胁迫下增加 ABA 水平来调节耐旱性。我们的研究表明，ABA 合成基因的转录水平也增加了。过表达 NtMLP423 减少了膜损伤和 ROS 积累，并在干旱胁迫下增加了应激相关基因的表达。我们还发现 NtWRKY71 调节 NtMLP423 的转录以提高耐旱性。

结论

我们的结果表明，NtMLP423 的过表达通过 ABA 途径提高了烟草的耐旱性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/4e8145c8a20e/12870_2020_2690_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/18ba268bf0d0/12870_2020_2690_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/f23dad3617c1/12870_2020_2690_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/31e3a903bf8f/12870_2020_2690_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/c21433a5f7bd/12870_2020_2690_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/f06cc52cd547/12870_2020_2690_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/229d691ded6e/12870_2020_2690_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/e5e49258a3b0/12870_2020_2690_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/733d379b3534/12870_2020_2690_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/4e8145c8a20e/12870_2020_2690_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/18ba268bf0d0/12870_2020_2690_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/f23dad3617c1/12870_2020_2690_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/31e3a903bf8f/12870_2020_2690_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/c21433a5f7bd/12870_2020_2690_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/f06cc52cd547/12870_2020_2690_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/229d691ded6e/12870_2020_2690_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/e5e49258a3b0/12870_2020_2690_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/733d379b3534/12870_2020_2690_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c30f/7565365/4e8145c8a20e/12870_2020_2690_Fig9_HTML.jpg

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