Tyrefors N, Gillberg P G
Department of Analytical Chemistry, Uppsala University, Sweden.
J Chromatogr. 1987 Dec 25;423:85-91. doi: 10.1016/0378-4347(87)80330-4.
A method for the determination of acetylcholine and choline in microdialysates from rat spinal cord has been developed using liquid chromatography, enzyme-catalysed post-column derivatization and electrochemical detection. The sensitivity required was achieved by a certain degree of miniaturization, careful temperature control and a slight modification of the amperometric detector. Determinations were performed on 5-microliter samples.
已开发出一种使用液相色谱、酶催化柱后衍生化和电化学检测来测定大鼠脊髓微透析液中乙酰胆碱和胆碱的方法。通过一定程度的小型化、精确的温度控制以及对安培检测器的轻微改进，实现了所需的灵敏度。对5微升的样品进行测定。
