Redpath Museum, McGill University, Montreal, Quebec, Canada.
Department of Biology, McGill University, Montreal, Quebec, Canada.
PLoS One. 2021 Aug 26;16(8):e0249439. doi: 10.1371/journal.pone.0249439. eCollection 2021.
We demonstrate that simple, non-invasive environmental DNA (eDNA) methods can detect transgenes of genetically modified (GM) animals from terrestrial and aquatic sources in invertebrate and vertebrate systems. We detected transgenic fragments between 82-234 bp through targeted PCR amplification of environmental DNA extracted from food media of GM fruit flies (Drosophila melanogaster), feces, urine, and saliva of GM laboratory mice (Mus musculus), and aquarium water of GM tetra fish (Gymnocorymbus ternetzi). With rapidly growing accessibility of genome-editing technologies such as CRISPR, the prevalence and diversity of GM animals will increase dramatically. GM animals have already been released into the wild with more releases planned in the future. eDNA methods have the potential to address the critical need for sensitive, accurate, and cost-effective detection and monitoring of GM animals and their transgenes in nature.
我们证明，简单的、非侵入性的环境 DNA（eDNA）方法可以从无脊椎动物和脊椎动物系统中的陆地和水生来源中检测到转基因动物的转基因。我们通过靶向 PCR 扩增从转基因果蝇（Drosophila melanogaster）的食物媒介、粪便、尿液和唾液以及转基因实验室小鼠（Mus musculus）的唾液以及转基因四齿鱼（Gymnocorymbus ternetzi）的水族馆水中提取的环境 DNA 来检测 82-234 bp 的转基因片段。随着 CRISPR 等基因编辑技术的快速普及，转基因动物的流行程度和多样性将大幅增加。已经有转基因动物被释放到野外，未来还计划有更多的释放。eDNA 方法有可能解决在自然界中对转基因动物及其转基因的敏感、准确和具有成本效益的检测和监测的迫切需求。
