Zhong Jing, Liang Yuxuan, Chen Yongchun, Zhang Jiawei, Zou Xiaoying, Deng Jie, Wang Da, Sun Yuanming, Li Meiying
College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Foods. 2021 Aug 12;10(8):1863. doi: 10.3390/foods10081863.
The function of Baroni (daylily) on promoting lactation is reported in several ancient Chinese medicine books. However, nowadays, there is no conclusive data to support this statement. In this study, we investigated the effect of Baroni extract (HCE) on lactation insufficiency in chronic unpredictable mild stress (CUMS) dams and further explored the mechanism and functional components through network pharmacology. The results showed that HCE could increase the offspring's weight, serum prolactin (PRL), and oxytocin (OT) level of CUMS dams. Network pharmacology analysis revealed that the facilitation of HCE on lactation is the result of the comprehensive action of 62 components on 209 targets and 260 pathways, among this network, quercetin, kaempferol, thymidine, etc., were the vital material basis, signal transducer and activator of transcription 3 (STAT3), mitogen activity protein kinase 1 (MAPK1), tumor protein P53 (TP53), etc., were the core targets, and the prolactin signaling pathway was the core pathway. In addition, verification test results showed that HCE regulated the abnormal expression of the prolactin signaling pathway, including STAT3, cyclin D1 (CCND1), MAPK1, MAPK8, nuclear factor NF-kappa-B p105 subunit (NFKB1), and tyrosine-protein kinase (JAK2). In conclusion, HCE exhibited a facilitation of lactation insufficiency, in which quercetin, kaempferol, thymidine, etc., were the most important material basis. The mechanism of this promotional effect is mediated by the prolactin signaling pathway in mammary gland.
几本中国古代医学典籍中记载了金针菜促进泌乳的作用。然而，如今尚无确凿数据支持这一说法。在本研究中，我们调查了金针菜提取物（HCE）对慢性不可预测轻度应激（CUMS）母鼠泌乳不足的影响，并通过网络药理学进一步探究其作用机制和功能成分。结果表明，HCE可增加CUMS母鼠后代的体重、血清催乳素（PRL）和催产素（OT）水平。网络药理学分析显示，HCE对泌乳的促进作用是62种成分作用于209个靶点和260条通路的综合结果，在该网络中，槲皮素、山奈酚、胸腺嘧啶核苷等是重要物质基础，信号转导和转录激活因子3（STAT3）、丝裂原活性蛋白激酶1（MAPK1）、肿瘤蛋白P53（TP53）等是核心靶点，催乳素信号通路是核心通路。此外，验证试验结果表明，HCE调节了催乳素信号通路的异常表达，包括STAT3、细胞周期蛋白D1（CCND1）、MAPK1、MAPK8、核因子NF-κB p105亚基（NFKB1）和酪氨酸蛋白激酶（JAK2）。综上所述，HCE对泌乳不足具有促进作用，其中槲皮素、山奈酚、胸腺嘧啶核苷等是最重要的物质基础。这种促进作用的机制是由乳腺中的催乳素信号通路介导的。
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