Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
The Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast BT7 1NN, UK.
Int J Mol Sci. 2023 Mar 2;24(5):4844. doi: 10.3390/ijms24054844.
Daylily ( Baroni) is an edible plant widely distributed worldwide, especially in Asia. It has traditionally been considered a potential anti-constipation vegetable. This study aimed to investigate the anti-constipation effects of daylily from the perspective of gastro-intestinal transit, defecation parameters, short-chain organic acids, gut microbiome, transcriptomes and network pharmacology. The results show that dried daylily (DHC) intake accelerated the defecation frequency of mice, while it did not significantly alter the levels of short-chain organic acids in the cecum. The 16S rRNA sequencing showed that DHC elevated the abundance of , and , while it reduced the level of pathogens (such as and ). Furthermore, a transcriptomics analysis revealed 736 differentially expressed genes (DEGs) after DHC treatment, which are mainly enriched in the olfactory transduction pathway. The integration of transcriptomes and network pharmacology revealed seven overlapping targets (, , , , , and ). A qPCR analysis further showed that DHC reduced the expression of Alb, Pon1 and Cnr1 in the colon of constipated mice. Our findings provide a novel insight into the anti-constipation effects of DHC.
萱草（Baroni）是一种广泛分布于世界各地的可食用植物，尤其在亚洲。它一直被认为是一种有潜在抗便秘作用的蔬菜。本研究从胃肠转运、排便参数、短链有机酸、肠道微生物组、转录组和网络药理学等方面探讨了萱草的抗便秘作用。结果表明，干萱草（DHC）摄入可加速小鼠的排便频率，而对盲肠内短链有机酸水平无显著影响。16S rRNA 测序显示，DHC 增加了 、 和 的丰度，而降低了病原体（如 和 ）的水平。此外，转录组分析显示，DHC 处理后有 736 个差异表达基因（DEGs），主要富集在嗅觉转导途径中。转录组和网络药理学的整合揭示了七个重叠的靶点（、、、、、和）。qPCR 分析进一步表明，DHC 降低了便秘小鼠结肠中 Alb、Pon1 和 Cnr1 的表达。我们的研究结果为 DHC 的抗便秘作用提供了新的见解。
