State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, PR China.
The Faculty of Engineering, Architecture and Information Technology, The University of Queensland, Brisbane QLD 4072, Australia.
Int J Biol Macromol. 2021 Oct 31;189:356-362. doi: 10.1016/j.ijbiomac.2021.08.145. Epub 2021 Aug 24.
As a crucial biomarker for some diseases, the determination of cholesterol in human serum is of great significance for the diagnosis and prevention of these diseases. Hence, a portable cholesterol detection method is necessary for clinical and domestic applications. Here, a portable paper sensor was designed for cholesterol detection by modifying screen-printed electrode (SPE) with nanoporous gold (NPG). To achieve the reliable cholesterol detection, a synergistic strategy was proposed based on the oxidation of cholesterol by cholesterol oxidase (ChOx) and the reduction of oxidation product (HO) by NPG. Compared to existing electrochemical sensors, the resulting paper sensor exhibited a wider linear response in a range from 50 μM to 6 mM as well as a higher sensitivity of 32.68 μA mM cm with a lower detection limit of 8.36 μM. Moreover, the portable paper sensor presented strong anti-interference capability and stability in the detection of cholesterol in human serum, and the data detected by the portable paper sensor were consistent with that obtained by an automatic biochemical analyzer. These unique performances confirmed that the proposed paper sensor was a sensitive, reliable, and portable cholesterol detection method, making it a good choice for cholesterol detection.
作为某些疾病的重要生物标志物，测定人血清中的胆固醇对于这些疾病的诊断和预防具有重要意义。因此，需要一种便携式胆固醇检测方法用于临床和家庭应用。本文设计了一种便携式纸基传感器，通过在丝网印刷电极（SPE）上修饰纳米多孔金（NPG）来实现胆固醇检测。为了实现可靠的胆固醇检测，提出了一种协同策略，基于胆固醇氧化酶（ChOx）氧化胆固醇和 NPG 还原氧化产物（HO）。与现有的电化学传感器相比，所制备的纸基传感器在 50μM 至 6mM 的范围内具有更宽的线性响应，灵敏度为 32.68μA·mM-1·cm-2，检测限低至 8.36μM。此外，该便携式纸基传感器在人血清中胆固醇检测具有较强的抗干扰能力和稳定性，且便携式纸基传感器检测到的数据与人自动生化分析仪获得的数据一致。这些独特的性能证实，所提出的纸基传感器是一种灵敏、可靠且便携式的胆固醇检测方法，是胆固醇检测的理想选择。
