Yu Huiyuan, Hu Mengjie, Wang Xiaolei, Wang Xia, Xun Luying, Liu Honglei
State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, China.
School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520, USA.
Molecules. 2024 Feb 28;29(5):1060. doi: 10.3390/molecules29051060.
Monitoring etoposide is important due to its wide usage in anti-tumor therapy; however, the commonly used HPLC method is expensive and often requires complicated extraction and detection procedures. Electrochemical analysis has great application prospects because of its rapid response and high specificity, sensitivity, and efficiency with low cost and high convenience. In this study, we constructed a nanoporous gold (NPG)-modified GCE for the detection of etoposide. The electrochemical oxidation of etoposide by NPG caused a sensitive current peak at +0.27 V with good reproductivity in 50 mM of phosphate buffer (pH 7.4). The relationship between etoposide concentration and peak current was linear in the range between 0.1 and 20 μM and between 20 and 150 μM, with a detection sensitivity of 681.8 μA mM cm and 197.2 μA mM cm, respectively, and a limit of detection (LOD) reaching 20 nM. The electrode had a good anti-interference ability to several common anions and cations. Spiked recovery tests in serum, urine, and fermentation broth verified the excellent performance of the sensor in terms of sensitivity, reproducibility, and specificity. This may provide a promising tool for the detection of etoposide in biological samples.
由于依托泊苷在抗肿瘤治疗中的广泛应用，对其进行监测很重要；然而，常用的高效液相色谱法成本高昂，且通常需要复杂的提取和检测程序。电化学分析因其响应迅速、特异性高、灵敏度高、效率高、成本低且便利性强而具有广阔的应用前景。在本研究中，我们构建了一种用于检测依托泊苷的纳米多孔金（NPG）修饰玻碳电极。NPG对依托泊苷的电化学氧化在50 mM磷酸盐缓冲液（pH 7.4）中于 +0.27 V处产生了一个灵敏的电流峰，且具有良好的重现性。依托泊苷浓度与峰电流之间的关系在0.1至20 μM以及20至150 μM范围内呈线性，检测灵敏度分别为681.8 μA mM cm和197.2 μA mM cm，检测限达到20 nM。该电极对几种常见阴离子和阳离子具有良好的抗干扰能力。在血清、尿液和发酵液中的加标回收试验验证了该传感器在灵敏度、重现性和特异性方面的优异性能。这可能为生物样品中依托泊苷的检测提供一种有前景的工具。
