Koppe Charlotte, Hoene Andreas, Walschus Uwe, Finke Birgit, Testrich Holger, Pohl Christopher, Brandt Nico, Patrzyk Maciej, Meichsner Jürgen, Nebe Barbara, Schlosser Michael
Department of General Surgery, Visceral, Thoracic and Vascular Surgery, University Medical Center Greifswald, 17487 Greifswald, Germany.
Leibniz Institute for Plasma Science and Technology (INP), 17487 Greifswald, Germany.
Polymers (Basel). 2021 Aug 11;13(16):2684. doi: 10.3390/polym13162684.
Orthopaedic implants and temporary osteosynthesis devices are commonly based on Titanium (Ti). For short-term devices, cell-material contact should be restricted for easy removal after bone healing. This could be achieved with anti-adhesive plasma-fluorocarbon-polymer (PFP) films created by low-temperature plasma processes. Two different PFP thin film deposition techniques, microwave (MW) and radiofrequency (RF) discharge plasma, were applied to receive smooth, hydrophobic surfaces with octafluoropropane (CF) or hexafluorohexane (CF) as precursors. This study aimed at examining the immunological local tissue reactions after simultaneous intramuscular implantation of four different Ti samples, designated as MW-CF, MW-CF, RF-CF and Ti-controls, in rats. A differentiated morphometric evaluation of the inflammatory reaction was conducted by immunohistochemical staining of CD68+ macrophages, CD163+ macrophages, MHC class II-positive cells, T lymphocytes, CD25+ regulatory T lymphocytes, NK cells and nestin-positive cells in cryosections of surrounding peri-implant tissue. Tissue samples were obtained on days 7, 14 and 56 for investigating the acute and chronical inflammation (n = 8 rats/group). Implants with a radiofrequency discharge plasma (RF-CF) coating exhibited a favorable short- and long-term immune/inflammatory response comparable to Ti-controls. This was also demonstrated by the significant decrease in pro-inflammatory CD68+ macrophages, possibly downregulated by significantly increasing regulatory T lymphocytes.
骨科植入物和临时骨固定装置通常以钛（Ti）为基础。对于短期装置，应限制细胞与材料的接触，以便在骨愈合后易于取出。这可以通过低温等离子体工艺制备的抗粘附等离子体氟碳聚合物（PFP）薄膜来实现。应用两种不同的PFP薄膜沉积技术，即微波（MW）和射频（RF）放电等离子体，以前体八氟丙烷（CF）或六氟己烷（CF）获得光滑的疏水表面。本研究旨在检查在大鼠肌肉内同时植入四种不同的钛样品（分别称为MW-CF、MW-CF、RF-CF和钛对照）后局部组织的免疫反应。通过对植入物周围组织冰冻切片中的CD68+巨噬细胞、CD163+巨噬细胞、MHC II类阳性细胞、T淋巴细胞、CD25+调节性T淋巴细胞、NK细胞和巢蛋白阳性细胞进行免疫组织化学染色，对炎症反应进行差异化形态计量学评估。在第7、14和56天获取组织样本，以研究急性和慢性炎症（每组n = 8只大鼠）。具有射频放电等离子体（RF-CF）涂层的植入物表现出与钛对照相当的良好短期和长期免疫/炎症反应。促炎CD68+巨噬细胞的显著减少也证明了这一点，这可能是由于调节性T淋巴细胞的显著增加而下调的。
