Hoene Andreas, Patrzyk Maciej, Walschus Uwe, Finke Birgit, Lucke Silke, Nebe Barbara, Schröder Karsten, Schlosser Michael
Department of Surgery, University Medical Center Greifswald, Greifswald, Germany.
J Mater Sci Mater Med. 2015 Mar;26(3):131. doi: 10.1007/s10856-015-5476-5. Epub 2015 Feb 20.
Implantation of biomaterials can cause complications often associated with inflammatory reactions. However, repeated evaluation of the implant site would be burdening for patients. Alternatively, blood examinations with analysis of inflammatory serum markers could potentially be useful to reflect the local cellular response for detection and/or prediction of inflammation-related complications. Therefore, following intramuscular implantation of surface-modified Ti implants in rats, this study aimed at examining possible associations between the post-implantation time course of pro-inflammatory (INFγ, IL-2) and anti-inflammatory (IL-4, IL-10) cytokine serum concentrations and the local peri-implant tissue response after 56 days (pro-inflammatory CD68-positive monocytes/macrophages, anti-inflammatory CD163-positive macrophages, MHC class II-positive cells, activated natural killer cells and mast cells). Multivariate correlation analysis revealed a significant interaction between serum IFNγ and peri-implant tissue CD68-positive monocytes/macrophages (p = 0.001) while no interactions were found for other cytokines and cell types. Additional Pearson correlation analysis of IFNγ serum concentrations on each experimental day vs. the CD68-positive monocytes/macrophages response on day 56 demonstrated a consistently positive correlation that was strongest during the first three weeks. Thus, high early pro-inflammatory IFNγ serum concentration was associated with high late number of pro-inflammatory CD68-positive monocyte/macrophages and low early serum IFNγ with low late CD68-positive monocyte/macrophage numbers. Further studies aimed at examination of patient samples could establish the relevance of this association to predict clinical complications. After implantation of titanium samples, high early IFNγ serum concentrations were associated with a pronounced late pro-inflammatory CD68-positive monocyte/ macrophage (red circle) response, while no correlation was found for other investigated cytokines and inflammatory cells (green circle). In contrast, low early IFNγ serum concentrations were correlated with low late monocyte/ macrophage numbers.
生物材料的植入可能引发通常与炎症反应相关的并发症。然而，对植入部位进行反复评估会给患者带来负担。另外，通过血液检查分析炎症血清标志物可能有助于反映局部细胞反应，以检测和/或预测炎症相关并发症。因此，在大鼠肌肉内植入表面改性钛植入物后，本研究旨在探讨促炎（INFγ、IL - 2）和抗炎（IL - 4、IL - 10）细胞因子血清浓度的植入后时间进程与56天后局部植入物周围组织反应（促炎CD68阳性单核细胞/巨噬细胞、抗炎CD163阳性巨噬细胞、MHC II类阳性细胞、活化自然杀伤细胞和肥大细胞）之间的可能关联。多变量相关性分析显示血清IFNγ与植入物周围组织CD68阳性单核细胞/巨噬细胞之间存在显著相互作用（p = 0.001），而其他细胞因子和细胞类型未发现相互作用。对每个实验日的IFNγ血清浓度与第56天CD68阳性单核细胞/巨噬细胞反应进行的额外Pearson相关性分析显示，在前三周相关性最强且始终呈正相关。因此，早期促炎IFNγ血清浓度高与晚期促炎CD68阳性单核细胞/巨噬细胞数量多相关，而早期血清IFNγ低与晚期CD68阳性单核细胞/巨噬细胞数量少相关。旨在检查患者样本的进一步研究可以确定这种关联对预测临床并发症的相关性。植入钛样本后，早期IFNγ血清浓度高与晚期明显的促炎CD68阳性单核细胞/巨噬细胞（红色圆圈）反应相关，而其他研究的细胞因子和炎症细胞（绿色圆圈）未发现相关性。相比之下，早期IFNγ血清浓度低与晚期单核细胞/巨噬细胞数量少相关。
