荧光标记影响阿拉伯木聚糖的结构/构象特性。
Fluorescent labeling affected the structural/conformational properties of arabinoxylans.
机构信息
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Research Centre of Modern Analysis Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.
出版信息
Carbohydr Polym. 2021 Aug 1;265:118064. doi: 10.1016/j.carbpol.2021.118064. Epub 2021 Apr 17.
Three fluorescent-labeling methods, including DMSO/DBTD, reductive amination and fluoresceinamine (FA), were applied to label arabinoxylan (AX). The conjugates were named AF, ATF and ACF, respectively. Compared to AX, the water solubility of AF significantly decreased while that of ATF significantly increased (p < 0.05); both showed decreased solubility with increasing degree of substitution (DS). However, AX was not successfully labeled by the FA method due to poor solubility. The structural and conformational properties of the AF and ATF conjugates under different DS were compared. Results showed that fluorochrome (FITC) was successfully grafted onto AX molecule by using the DMSO/DBTD and reductive amination methods. A slight increased Mw was noticed for both AF and ATF. AF existed in a more compact conformation, while the chain conformation of ATF was more elongated at a lower DS (1.15 %) and more compact at a higher DS (1.34 %).
三种荧光标记方法，包括 DMSO/DBTD、还原胺化和荧光胺（FA），被应用于阿拉伯木聚糖（AX）的标记。相应的缀合物分别命名为 AF、ATF 和 ACF。与 AX 相比，AF 的水溶性显著降低，而 ATF 的水溶性显著增加（p < 0.05）；随着取代度（DS）的增加，两者的水溶性均降低。然而，由于溶解度差，FA 方法未能成功标记 AX。比较了不同 DS 下 AF 和 ATF 缀合物的结构和构象特性。结果表明，通过 DMSO/DBTD 和还原胺化方法，成功地将荧光染料（FITC）接枝到 AX 分子上。AF 和 ATF 的重均分子量（Mw）均略有增加。AF 存在更紧凑的构象，而 ATF 的链构象在较低 DS（1.15%）时更长，在较高 DS（1.34%）时更紧凑。
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