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慢性伤口临床分离株的检测与鉴定

Detection and Characterization of Clinical Isolates from Chronic Wounds.

作者信息

Buechler Christian, Neidhöfer Claudio, Hornung Thorsten, Neuenhoff Marcel, Parčina Marijo

机构信息

Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany.

Clinic and Polyclinic for Dermatology and Allergology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany.

出版信息

Pathogens. 2021 Jul 30;10(8):966. doi: 10.3390/pathogens10080966.

Abstract

is a relatively newly discovered and potentially frequently overlooked species, mostly isolated from chronic wounds and predominantly in those of the lower extremities. Its susceptibility profile and clinical significance is still debated, given the limited amount of available data. We contribute providing a molecular and phenotypical analysis of three unique clinical isolates detected between August 2019 and January 2020 to aid the matter. Cryo-conserved isolates were subcultured and re-identified using various routine means of identification. Bacterial genomes were fully Illumina-sequenced and phenotypical susceptibility was determined by broth microdilution and gradient-strip tests. All isolates displayed increased susceptibility to piperacillin-tazobactam (<2/4 mg/L), imipenem (<1 mg/L), and meropenem (<0.047 mg/L), whereas they displayed decreased susceptibility to all tested cephalosporins and fluoroquinolones (according to PK-PD, EUCAST 10.0 2020). One isolate carried a beta-lactamase (EC 3.5.2.6) and a sulfonamide resistance gene (sul2) and cells displayed resistance to ampicillin, ampicillin/sulbactam, and trimethoprim/sulfamethoxazole. All isolates carried genes conferring decreased susceptibility to aminoglycosides (aadA), fosfomycin (fosA) and fluoroquinolones (gyrB EC 5.99.1.3). Awareness that can be resistant to trimethoprim/sulfamethoxazole is warranted.

摘要

是一种相对新发现且可能经常被忽视的物种,主要从慢性伤口中分离出来,且主要来自下肢伤口。鉴于可用数据有限,其药敏谱和临床意义仍存在争议。我们通过对2019年8月至2020年1月期间检测到的三株独特临床分离株进行分子和表型分析,以推动该问题的解决。对冷冻保存的分离株进行传代培养,并使用各种常规鉴定方法重新鉴定。对细菌基因组进行全基因组Illumina测序,并通过肉汤微量稀释法和梯度条试验确定表型药敏性。所有分离株对哌拉西林-他唑巴坦(<2/4 mg/L)、亚胺培南(<1 mg/L)和美罗培南(<0.047 mg/L)的敏感性增加,而对所有测试的头孢菌素和氟喹诺酮类药物的敏感性降低(根据PK-PD,EUCAST 10.0 2020)。一株分离株携带β-内酰胺酶(EC 3.5.2.6)和磺胺耐药基因(sul2),细胞对氨苄西林、氨苄西林/舒巴坦和甲氧苄啶/磺胺甲恶唑耐药。所有分离株都携带赋予对氨基糖苷类(aadA)、磷霉素(fosA)和氟喹诺酮类(gyrB EC 5.99.1.3)敏感性降低的基因。有必要认识到[该物种]可能对甲氧苄啶/磺胺甲恶唑耐药。

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